5.3.3.3	Fe-S	0.8 mol Fe and 10.8 mol S per mol of enzyme, iron-sulfur protein, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity	
5.3.3.3	Fe-S	16 mol Fe and 14 mol S per mol of enzyme, iron-sulfur protein, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity	
5.3.3.3	additional information	not influenced by Na+ or K+	
5.3.3.3	additional information	no activation by Fe2+, Mn2+, Co2+, Ni2+, Ca2+, Mg2+, Zn2+