4.2.3.12	Ba2+	stimulatory effect, 5 mM greatly accelerates activity	648561	
4.2.3.12	Ca2+	65% of the activity with equal concentration of Mg2+	648564	
4.2.3.12	Ca2+	less than 15% of activity with Mg2+ with 5 mM	648554	
4.2.3.12	Ca2+	stimulatory effect and additive effect at Mg2+, 1 mM	648561	
4.2.3.12	Co2+	free metal enzyme activity reaches 60% of activity of wild type with 0.50 mM and concentrations higher than 0.15 mM completely inactivates the enzyme. Less than 15% of activity with Mg2+ with 5 mM	648554	
4.2.3.12	Mg2+	75% enhancement	716147	
4.2.3.12	Mg2+	free metal enzyme activity is 15% of the wild type with 8 mM, it is not bound to the enzyme	648554	
4.2.3.12	Mg2+	necessary for activity	648555, 648559	
4.2.3.12	Mg2+	optimum concentration is 8 mM	648564	
4.2.3.12	Mg2+	required	648553, 648555	
4.2.3.12	Mg2+	stimulates, Km of 0.2 mM and 2 mM at pH 8.5 and 37ºC	648561	
4.2.3.12	Mn2+	less than 15% of activity with Mg2+ with 5 mM	648554	
4.2.3.12	Mn2+	stimulatory effect but additional competitive or inhibitory effect	648561	
4.2.3.12	additional information	Cu2+ has no effect on activity	648561	
4.2.3.12	additional information	Cu2+, Fe2+, less than 15% of activity with Mg2+ with 5 mM of each ion	648554	
4.2.3.12	additional information	no metal ion required	648568	
4.2.3.12	Ni2+	71% of the activity with Mg2+ with NiCl2, 5 mM	648554	
4.2.3.12	Ni2+	no effect on enzyme activity	648561	
4.2.3.12	Sr2+	5 mM greatly accelerates activity	648561	
4.2.3.12	Zn2+	-	648553, 702280, 704296	
4.2.3.12	Zn2+	bound to the enzyme	648554	
4.2.3.12	Zn2+	bound to the Nepsilon-atoms of the three His residues HisA23, HisA48, and HisA50, one to each of the two active sites	648560	
4.2.3.12	Zn2+	concentrations higher than 0.15 mM completely inactivates the enzyme	648554	
4.2.3.12	Zn2+	free metal enzyme activity reaches 85% of activity of wild type with 0.05 mM and 8 mM Mg2+	648554	
4.2.3.12	Zn2+	no effect on enzyme activity	648561	
4.2.3.12	Zn2+	the zinc/enzyme-subunit ratio of the wild-type enzyme is 0.8	648554