4.2.1.2	Ca2+	has a small stimulatory effect	715093	
4.2.1.2	Fe	enzyme contains an oxygen-sensitive [4Fe-4S] cluster	679910	
4.2.1.2	Fe2+	aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol	749072	
4.2.1.2	Fe2+	storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol	746828	
4.2.1.2	Iron	fumarase A contains a catalytically active [4Fe-4S]cluster	33770	
4.2.1.2	Iron	in cells grown without aeration the Fe-S-independent enzyme form occupies over 80% of the overall fumarase. In aerobically grown cells the Fe-S-dependent fumarase occupies 80% of the overall activity	33747	
4.2.1.2	Iron	one Fe-S-dependent enzyme form and one Fe-S-independent enzyme form	33747, 33768	
4.2.1.2	Iron	[4Fe-4S]-cluster containing heterodimeric enzyme	745719	
4.2.1.2	Mg2+	10fold activation of isoform Fum2	749006	
4.2.1.2	Mg2+	2 mM, slight stimulation	678771	
4.2.1.2	Mg2+	about 20fold activation of isoform Fum1	749006	
4.2.1.2	Mg2+	cytosolic form gets strongly activated, mitochondrial form only moderately activated	730558	
4.2.1.2	Mg2+	required, stimulates 10% at 5 mM	716122	
4.2.1.2	Mn2+	15fold activation of isoform Fum2	749006	
4.2.1.2	Mn2+	cytosolic form gets strongly activated, mitochondrial form only less effectively activated	730558	
4.2.1.2	additional information	activity does not increase upon incubation with Fe2+	749072	
4.2.1.2	additional information	aluminium and gallium do not perturb the activity of fumarase C	695741	
4.2.1.2	additional information	class II fumarase activity is frequently influenced by divalent metal ions and non-ionic surfactants. No effect by Ca2+	716122	
4.2.1.2	additional information	no effects of Zn2+, Fe2+, or EDTA on enzyme activity	715093	
4.2.1.2	additional information	not activating: Ca2+, K+	749006	
4.2.1.2	additional information	not activating: Ca2+, K+, Mn2+	749006	
4.2.1.2	[3Fe-4S] center	10-25% of purified protein contains [3Fe-4S] cluster	730721