4.2.1.114 Fe2+ the enzyme contains an iron-sulfur center, reconstitution of the purified recombinant apoenzyme with iron-sulfur, overview 4.2.1.114 Fe2+ required, in a Fe-S cluster 4.2.1.114 Iron-sulfur cluster the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site 4.2.1.114 Mg2+ required for the hydratase reaction, not for the hydrolase reaction