4.2.1.108 Fe2+ required, activates 100fold at 1 mM 4.2.1.108 additional information the enzyme is not affected by Mg2+, Ca2+, Mn2+, Li+, K+, and NH4+ 4.2.1.108 additional information determination of metal content of recombinant SaEctC protein by ICP-MS. Zn2+ or Co2+ can only weakly substitute for Fe2+. No activation with Ni2+, Mn2+, Cu2+, and Fe3+ at 1 mM 4.2.1.108 NaCl required for activity 4.2.1.108 NaCl required for activity, maximal activity at 0.5 M