3.6.1.41 Mn2+ 900fold stimulation 246770 3.6.1.41 Mn2+ best activator. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 733872 3.6.1.41 Ni2+ 0.004-0.008 mM, 3.5fold stimulation 246765