3.5.5.5	Mg2+	stimulates hydrolytic activity to 122%	
3.5.5.5	additional information	does not contain one of the following elements: Be, B, Mg, Al, Si, P, S, Cs, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Se, Sr, Zr, Mo, Pd, Ag, Cd, Sn, Sb, Ba, Ta, W, Pt, Au, Hg, Pb, La or Ce	
3.5.5.5	additional information	enzyme does not seem to require metals for activity	
3.5.5.5	additional information	no effects by Ca2+ and EDTA at 5 mM	
3.5.5.5	additional information	Zn2+, Na+, and K+ show very little effect on arylacetonitrilase activity