3.5.4.16	Fe2+	dependent, contains 1 Fe2+ per protomer	685163	
3.5.4.16	K+	activates	209550, 209564	
3.5.4.16	Li+	activates	209550	
3.5.4.16	Mg2+	activates	209546, 209566	
3.5.4.16	Mn2+	at 2 mM Mn2+ is able to restore MptA activity to a higher level than Fe2+, Mn2+ enzyme is not inactivated by exposure to oxygen	685163	
3.5.4.16	Mn2+	required for activity of isozyme GCYH-1B	712290	
3.5.4.16	additional information	no metals required	209552, 209553	
3.5.4.16	Na+	activates	209550	
3.5.4.16	Zn2+	1 ion per active site	656261	
3.5.4.16	Zn2+	absolutely required, e.g. for activity and reconstitution of the denatured enzyme, 1 Zn2+ bound per subunit of the decameric enzyme	655489	
3.5.4.16	Zn2+	catalytically essential ion, complexed to 2 cysteine and 1 histidine residue	654607	
3.5.4.16	Zn2+	essential for activity, coordinated to a His-side chain of the active site	657176	
3.5.4.16	Zn2+	essential for activity, coordinated to a His-side chain of the active site, mechanistic role	657176	
3.5.4.16	Zn2+	essential, bound to 2 Cys and 1 His	657188	
3.5.4.16	Zn2+	isozyme GCYH-1A is a Zn2+-dependent enzyme, isozyme GCYH-IB is expressed only under Zn2+-limiting conditions	712290	
3.5.4.16	Zn2+	required, wild-type enzyme contains 0.9 mol of Zn2+ per mol of enzyme subunit, while the zinc content of the mutant enzymes is reduced to below 0.2 Zn2+ per mol of enzyme subunit	656511	
3.5.4.16	Zn2+	the zinc ion is bound by residues Cys108, His111, and Cys179	669149	
3.5.4.16	Zn2+	Zn2+-dependent enzyme	756001