3.5.1.19	Ca2+	activation	246576	
3.5.1.19	Co2+	addition of Co2+ after metal removement results in 13% activity	718883	
3.5.1.19	Co2+	enhances activity	246588	
3.5.1.19	Fe2+	-	679806	
3.5.1.19	Fe2+	activates	710862	
3.5.1.19	Fe2+	addition of Fe2+ after metal removement results in 13% activity	718883	
3.5.1.19	Fe2+	the conserved specific metal ion binding includes one aspartate (D67) and two histidines (H69 and H86)	753679	
3.5.1.19	Fe2+	the enzyme contains 0.47 M of Fe2+ per mol of enzyme	718885	
3.5.1.19	Fe2+	the enzyme contains a Fe2+ ion in its metal binding site, which is coordinated by an aspartate (D67) and three histidine residues (H69, H75 and H86)	755150	
3.5.1.19	Fe2+	the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+	729184	
3.5.1.19	Fe2+	the enzyme contains Fe2+ in the active site	727053	
3.5.1.19	Mg2+	activation	246576	
3.5.1.19	Mg2+	required	246567, 711827	
3.5.1.19	Mn2+	-	679806	
3.5.1.19	Mn2+	activates	710862	
3.5.1.19	Mn2+	activation	246576	
3.5.1.19	Mn2+	addition of Mn2+ after metal removement results in 27% activity	718883	
3.5.1.19	Mn2+	enhances activity	246588	
3.5.1.19	Mn2+	the enzyme contains 0.44 M Mn2+ per mol of enzyme	718885	
3.5.1.19	additional information	addition of Fe3+ or Ni2+ after metal removement results in no detectable activity	718883	
3.5.1.19	additional information	PncA is a divalent cation-dependent enzyme	710862	
3.5.1.19	additional information	the enzyme contains 0.235 mM of Zn2+ in 0.242 mM protein, while it only contains 0.0032 mM of Fe2+ and 0.00077 mM Mn2+	735084	
3.5.1.19	Ni2+	enhances activity	246588	
3.5.1.19	Zn2+	-	677824	
3.5.1.19	Zn2+	activates, best cation, the active site Cys159 is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site	710862	
3.5.1.19	Zn2+	contains tightly bound zinc. Addition of 2 mM ZnCl2 fails to increase the activity of the wild type enzyme	718924	
3.5.1.19	Zn2+	contains zinc, highest level of activity in the presence of Zn2+	718883	
3.5.1.19	Zn2+	presence of Zn2+ in the active center of the enzyme in a molecular ratio of 1:1 of protein to metal ion	735084	
3.5.1.19	Zn2+	zinc-dependent enzyme	734741