3.2.1.2 2-mercaptoethanol activates 208635 3.2.1.2 Ag+ activates 12% at 10 mM 750376 3.2.1.2 Ba2+ stimulates 208604 3.2.1.2 BaCl2 1 mM, activates 654141 3.2.1.2 Ca2+ 100 mM CaCl2, 29% stimulation 208622 3.2.1.2 Ca2+ activates 663558, 717950, 752021 3.2.1.2 Ca2+ activates 11% at 10 mM 750376 3.2.1.2 Ca2+ activity is increased to 150 % in the presence of calcium chloride 746791 3.2.1.2 Ca2+ one Ca2+ is bound per molecule of enzyme far from the active site 208645 3.2.1.2 Ca2+ stimulates 208604 3.2.1.2 Ca2+ strong stimulation 730278 3.2.1.2 CaCl2 1 mM, activates 654141 3.2.1.2 CoCl2 1 mM, activates 654141 3.2.1.2 cysteine activates 208635 3.2.1.2 dithiothreitol activates 208635 3.2.1.2 K+ 100 mM, 20-30% stimulation 208622 3.2.1.2 K+ activates 10% at 10 mM 750376 3.2.1.2 K+ stimulates 208604 3.2.1.2 Mg2+ activates, maximum enzyme activity of 925.4 U/ml is achieved in the presence of 50 mM Mg2+ 752021 3.2.1.2 MnCl2 1 mM, activates 654141 3.2.1.2 additional information no requirement for metal ions 208602 3.2.1.2 additional information the presence or absence of various divalent cations or EDTA had a minimal effect on enzyme activity 721339 3.2.1.2 Na+ activates 11% at 10 mM 750376 3.2.1.2 NaCl 100 mM, 28% stimulation 208622 3.2.1.2 NaCl activates, best at 10-12% activity drops off dramatically at low NaCl concentrations 717950 3.2.1.2 NaCl optimum concentration 12.5% 730278 3.2.1.2 SrCl2 1 mM, activates 654141