2.8.2.8 Ca2+ 22% of the activity with Mn2+ 645930 2.8.2.8 Ca2+ 41% of the activity with Mn2+ 645926 2.8.2.8 Ca2+ can partially replace Mn2+ in activation 645934 2.8.2.8 Mg2+ 52% of the activity with Mn2+ 645926 2.8.2.8 Mg2+ activates at 10 mM 645933 2.8.2.8 Mg2+ little effect 645934 2.8.2.8 Mg2+ metal ion required, maximal activity with 5 mM 645930 2.8.2.8 Mn2+ 10 mM, 4 to 5fold activation 645934 2.8.2.8 Mn2+ 62% of the activity with Mg2+ 645930 2.8.2.8 Mn2+ divalent cation required 645926, 645934 2.8.2.8 Mn2+ maximal activation at: 5 mM 645926 2.8.2.8 Mn2+ Mn2+ most effective 645926, 645934 2.8.2.8 Mn2+ required 661838, 760624 2.8.2.8 additional information divalent metal ions are not required for N-sulfotransferase activity of NDST-1 738657 2.8.2.8 additional information greatest activity in presence of sodium phosphate buffer, in imidazole-HCl reaction rate is lower 645933 2.8.2.8 additional information no metal ions required 645938