2.7.4.14 Ca2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) 645139 2.7.4.14 Co2+ divalent cation required, Mn2+ can substitute for Mg2+ 645149 2.7.4.14 Mg2+ - 673598, 692040, 693063, 694478, 694479 2.7.4.14 Mg2+ divalent cation required, Mg2+ is most effective 645149 2.7.4.14 Mg2+ optimal concentration is 2-3 mM 645141 2.7.4.14 Mg2+ required 737991, 739215, 739700 2.7.4.14 Mg2+ required for phosphorylation of CMP, UMP and dCMP by either ATP or dCTP 645143 2.7.4.14 Mg2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) 645139 2.7.4.14 Mg2+ strong requirement, maximal activity at 3 mM 645160 2.7.4.14 Mn2+ divalent cation required, Mn2+ can substitute for Mg2+ 645149 2.7.4.14 Mn2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) 645139 2.7.4.14 Mn2+ with CMP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+, 100%, Mn2+, 42%, Ni2+, 15% and Ca2+, 13% 645143 2.7.4.14 Na2SO4 250 mM, stimulates 645139 2.7.4.14 NaCl 250 mM, stimulates 645139 2.7.4.14 Ni2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) 645139