2.7.11.14 Ca2+ affects enzyme binding to rhodopsin, induces binding of recoverin to the enzyme inhibiting its activity, NMR structure determination and analysis of the ternary complex RK25-Ca2+-recoverin 674709 2.7.11.14 Ca2+ Ca2+ controls the binding of recoverin to the enzyme 760605 2.7.11.14 Ca2+ Ca2+-dependent phosphorylation of arrestin, Ca2+ concentrations above 0.0025 mM free Ca2+ stimulate, maximal at 0.05 mM Ca2+, Ca2+ may facilitate arrestin-binding to RK, phosphorylation of rhodopsin is not Ca2+-dependent 640713 2.7.11.14 Ca2+ controls the binding of recoverin to the enzyme 664267 2.7.11.14 Ca2+ dependent on, rhodopsin phosphorylation in rod outer segment membranes 665584 2.7.11.14 Ca2+ enzyme activity requires light-activation, Ca2+ and membranes 675513 2.7.11.14 Ca2+ GRK1 activity is inhibited by binding of the Ca2+-bound form of S-modulin/recoverin 689386 2.7.11.14 Ca2+ induces a conformational change, while in the calciumfree form, the myristoyl group is held within a hydrophobic cleft, addition of calcium leads to its release and to membrane binding, inhibits rhodopsin binding to the enzyme via recoverin, which binds to the amphipathic peptide at the N-terminus of the enzyme and blocks rhodopsin binding 674596 2.7.11.14 Ca2+ regulation of the rhodopsin activity by recoverin and calmodulin is Ca2+-dependent 725081 2.7.11.14 Mg2+ - 490797, 490997, 491015, 640709, 640710, 640711, 640712, 640713, 640714, 640716, 640717, 640718, 640719, 640720, 640722, 640723, 640724, 640725, 640726, 640727, 640728, 640729, 640730, 662393, 663926, 664267, 665584, 666708, 674596, 675513, 675527, 676271, 687706, 689386 2.7.11.14 Mg2+ 1 mM required 640717 2.7.11.14 Mg2+ actual substrate: Mg-ATP complex, plus activation by free Mg2+-ions 640703 2.7.11.14 Mg2+ inhibition at high concentrations 640699 2.7.11.14 Mg2+ inhibitory in excess, presumably by formation of Mg-ATP-Mg 640703 2.7.11.14 Mg2+ requirement 490996, 640695, 640696, 640697, 640698, 640699, 640700, 640701, 640702, 640703, 640704, 640705, 640706, 640708, 640717 2.7.11.14 Mg2+ the optimum activation is obtained with a 10:1 ratio of Mg2+/ATP 640703 2.7.11.14 additional information Ca2+-independent phosphorylation of rhodopsin 640726