2.4.1.347 KCl upon an increase in the KCl concentration from 0 to 100 mM, the OtsA activity decreases by more than 40%, whereas it is not significantly affected when UDP or GDP-glucose is used as the substrate 2.4.1.347 Mg2+ 10 mM, required for maximal activity 2.4.1.347 Mg2+ stimulation, maximum activity at 2 mM. In absence of Mg2+, 36% of maximum activity 2.4.1.347 Mn2+ enzyme activity is increased about 2fold by 10 mM Mn2+ 2.4.1.347 NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10-30%