2.4.1.266 Co2+ and Mn2+, stimulation 732910 2.4.1.266 Co2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM 680455 2.4.1.266 Co2+ strictly dependent on divalent cations, in the following order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ 674318 2.4.1.266 Co2+ the recombinant glucosyl-3-phosphoglycerate synthase (GpgS) is dependent on divalent cations for activity: Co2+, Mn2+, Ni2+, Mg2+, and Zn2+. Co2+ (5 mM) has a more pronounced stimulatory effect 708933 2.4.1.266 Mg2+ - 680490 2.4.1.266 Mg2+ activity is strictly dependent on Mg2+, maximal activation at 20 mM 692382 2.4.1.266 Mg2+ activity is strictly dependent on Mg2+, maximal activation at 20-75 mM 692382 2.4.1.266 Mg2+ GpgS is dependent on divalent cations in the decreasing order of efficiency: Mg2+ (20 mM) > Mn2+ (10 mM) 759741 2.4.1.266 Mg2+ maximum activtiy in presence of 1 mM Mg2+ 732910 2.4.1.266 Mg2+ strictly dependent on 692382 2.4.1.266 Mg2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+ 680455 2.4.1.266 Mg2+ strictly dependent on divalent cations, in the following order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ 674318 2.4.1.266 Mg2+ the recombinant glucosyl-3-phosphoglycerate synthase (GpgS) is dependent on divalent cations for activity: Co2+, Mn2+, Ni2+, Mg2+, and Zn2+. Co2+ (5 mM) has a more pronounced stimulatory effect 708933 2.4.1.266 Mn2+ and Co2+, stimulation 732910 2.4.1.266 Mn2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM 680455 2.4.1.266 Mn2+ strictly dependent on divalent cations, in the following order of efficiency: Mn2+, Co2+, Mg2+, Ni2+. The maximum activation is obtained with 1.0 mM Mn2+. 50% of the maximum activity is reached with 0.19 mM Mn2+ 674318 2.4.1.266 Mn2+ the recombinant glucosyl-3-phosphoglycerate synthase (GpgS) is dependent on divalent cations for activity: Co2+, Mn2+, Ni2+, Mg2+, and Zn2+. Co2+ (5 mM) has a more pronounced stimulatory effect 708933 2.4.1.266 additional information K+, Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate GpgS activity at any concentration 674318, 680455 2.4.1.266 Ni2+ strictly dependent on divalent cations, in the following order of efficiency: Mn2+, Co2+, Mg2+, Ni2+. The specific activities obtained with Ni2+ are similar for both the His-tagged enzyme and the nontagged recombinant enzyme 674318 2.4.1.266 Ni2+ the recombinant glucosyl-3-phosphoglycerate synthase (GpgS) is dependent on divalent cations for activity: Co2+, Mn2+, Ni2+, Mg2+, and Zn2+. Co2+ (5 mM) has a more pronounced stimulatory effect 708933 2.4.1.266 Zn2+ the recombinant glucosyl-3-phosphoglycerate synthase (GpgS) is dependent on divalent cations for activity: Co2+, Mn2+, Ni2+, Mg2+, and Zn2+. Co2+ (5 mM) has a more pronounced stimulatory effect 708933