2.4.1.149	Cd2+	CdCl2 slightly stimulates	489473	
2.4.1.149	Mg2+	MgCl2 slightly stimulates	489473	
2.4.1.149	Mn2+	MnCl2: 10-25 mM for optimal activity required	489473	
2.4.1.149	Mn2+	requirement	638561	
2.4.1.149	Mn2+	essential for activity, neither Mg2+ nor Ca2+ can substitute for Mn2+	638575	
2.4.1.149	additional information	not activated by Mg2+ or Ca2+	638575	
2.4.1.149	Mn2+	strict requirement	638576, 638578	
2.4.1.149	Mn2+	MnCl2 stimulates 2.5fold, optimal concentration: 20 mM	638577	
2.4.1.149	additional information	absolute requirement for divalent cations, not stimulated by Mg2+	638577	
2.4.1.149	Co2+	slightly stimulates, 12.8% as effective as Mn2+ at 10 mM	638578	
2.4.1.149	Mn2+	optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+	638578	
2.4.1.149	additional information	enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+	638578	
2.4.1.149	Ca2+	activates, 48% as effective as Mn2+	638606	
2.4.1.149	Co2+	activates, 52% as effective as Mn2+	638606	
2.4.1.149	Mn2+	requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+	638606	
2.4.1.149	additional information	not activated by Mg2+, Zn2+, Cu2+ or Cd2+	638606	
2.4.1.149	Ca2+	about one third of the activity compared to Mn2+, recombinant enzyme	658728	
2.4.1.149	Mg2+	about one third of the activity compared to Mn2+, recombinant enzyme	658728	
2.4.1.149	Mn2+	required for activity, recombinant enzyme	658728