2.4.1.101 Ba2+ no activation 488360 2.4.1.101 Ba2+ slight activation 488368, 488370 2.4.1.101 Ca2+ - 488370 2.4.1.101 Ca2+ can substitute Mn2+ 488371 2.4.1.101 Ca2+ slight activation 488360, 488368, 488370 2.4.1.101 Ca2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective 658192 2.4.1.101 Cd2+ 78% efficiency 488360 2.4.1.101 Cd2+ activation 488360, 488368, 488370 2.4.1.101 Cd2+ can substitute for Mn2+ with 20% 488368 2.4.1.101 Co2+ 91% efficiency 488360 2.4.1.101 Co2+ activation 488360, 488368, 488370 2.4.1.101 Co2+ can substitute for Mn2+ with 80% 488368 2.4.1.101 Co2+ Co2+, Mn2+ or Mg2+ required, Co2+ is most effective 659000 2.4.1.101 Co2+ requirement 488379 2.4.1.101 Co2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective 658192 2.4.1.101 Fe2+ activation 488360, 488368, 488370 2.4.1.101 Fe2+ can substitute for Mn2+ with 65% efficiency 488368 2.4.1.101 Fe2+ slight 488360 2.4.1.101 Mg2+ 61% efficiency 488360 2.4.1.101 Mg2+ activation 488360, 488368, 488370 2.4.1.101 Mg2+ can substitute for Mn2+ with 40% 488368 2.4.1.101 Mg2+ Co2+, Mn2+ or Mg2+ required 659000 2.4.1.101 Mg2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective 658192 2.4.1.101 Mn2+ - 488381 2.4.1.101 Mn2+ 18-26 mM 488362 2.4.1.101 Mn2+ 2 mM 488363 2.4.1.101 Mn2+ 20-80 mM 488372 2.4.1.101 Mn2+ 25-100 mM 488370 2.4.1.101 Mn2+ binds not independently of UDP-N-acetyl-D-glucose 488376 2.4.1.101 Mn2+ can also use Ca2+ 488371 2.4.1.101 Mn2+ Co2+, Mn2+ or Mg2+ required, optimal concentration is 50 mM 659000 2.4.1.101 Mn2+ maximal activity in the presence of 2 mM Mn2+ 722834 2.4.1.101 Mn2+ required 760144 2.4.1.101 Mn2+ required for activity 721884 2.4.1.101 Mn2+ requirement 394591, 488360, 488361, 488362, 488363, 488364, 488365, 488366, 488368, 488370, 488371, 488372, 488379 2.4.1.101 Mn2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective 658192 2.4.1.101 additional information no activation by Cu2+ 488360, 488368, 488370 2.4.1.101 additional information no activation by Hg2+ 488360 2.4.1.101 additional information no activation by Sr2+, Pb2+ 488368, 488370 2.4.1.101 additional information not stimulated by Mg2+, Co2+, Ca2+, Zn2+ and C2+ 722834 2.4.1.101 Ni2+ 34% efficiency 488360 2.4.1.101 Ni2+ activation 488360, 488368, 488370 2.4.1.101 Ni2+ can substitute for Mn2+ with 20% 488368 2.4.1.101 Sn2+ slight activation 488368, 488370 2.4.1.101 Zn2+ 19% efficiency 488360 2.4.1.101 Zn2+ activation 488360, 488368, 488370 2.4.1.101 Zn2+ can substitute for Mn2+ with 8% 488368