2.1.1.182 KCl slightly stimulated up to 0.2 M 2.1.1.182 Mg2+ required 2.1.1.182 Mg2+ 2-3 mM, required 2.1.1.182 Mg2+ methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate 2.1.1.182 additional information K+ and NH4+ in the range of 10 to 150 mM have little effect on methylation 2.1.1.182 NaCl slightly stimulated up to 0.2 M