1.2.3.4 AlCl3 the activity of oxalate oxidase and the production of H2O2 in the root border colls is higher in Al-treated root tips relative to those of the control plants 676476 1.2.3.4 Ca2+ stimulates activity 741317 1.2.3.4 Cu2+ - 288389, 288390, 288391 1.2.3.4 Cu2+ 0.015 g per mol protein 288378 1.2.3.4 Cu2+ 0.04 atoms per monomer 288383 1.2.3.4 Cu2+ 14% increased activity at 10 mM Cu2+ 684408 1.2.3.4 Cu2+ 21% increase of activity at 1 mM 725764 1.2.3.4 Cu2+ 76% stimulation of activity at 0.5 mM Cu2+ 725122 1.2.3.4 Fe2+ 0.015 g per mol protein 288378 1.2.3.4 Fe2+ 0.09 atoms per subunit 288392 1.2.3.4 Fe2+ 0.87 atoms per monomer 288383 1.2.3.4 Fe2+ 10% increase of activity at 1 mM 725764 1.2.3.4 Fe2+ stimulates activity 741317 1.2.3.4 Mg2+ 40% increased activity at 10 mM Mg2+ 684408 1.2.3.4 Mg2+ Soluble and active protein obtained only when the enzyme is coexpressed with the chaperones DnaK and DnaJ (G isoform) and when a manganese salt is added to the growth medium (C and G isoform) 671415 1.2.3.4 Mn2+ - 687082 1.2.3.4 Mn2+ 0.201 g per monomer, 6-coordination 288378 1.2.3.4 Mn2+ 0.41 atoms per monomer 288383 1.2.3.4 Mn2+ 1.12 atoms per subunit 288392 1.2.3.4 Mn2+ 37% increase of activity at 1 mM 725764 1.2.3.4 Mn2+ 40% increased activity at 10 mM Mn2+ 684408 1.2.3.4 Mn2+ absolutely required 763395 1.2.3.4 Mn2+ contains between 0.1 and 0.4 mole Mn per mole enzyme 724114 1.2.3.4 Mn2+ dependent on 726327, 739991, 739993 1.2.3.4 Mn2+ titration of periodate-oxidized oxalate oxidase with hydroxylamine completely eliminates the visible absorption, forming a homogeneous Mn2+ form of the enzyme. The fully reduced Mn2+ form lacks any detectable oxidase activity, reoxidation substantially restores the maximum activity. 674894 1.2.3.4 Mn3+ treatment of the periodate-oxidized enzyme with ascorbate results in a substantioal decrease in absorption, forming a complex that is spectroscopically identified as a Mn3+ species. Mn3+ form has a 5fold higher specific activity than native recombinant oxalate oxidase. 674894 1.2.3.4 Mn5+ titration of oxalate oxidase with sodium periodate results in nearly stoichometric oxidation of the enzyme to an intensely colored yellow complex, whose complete spectroscopic characterization lead to assignment to a superoxidized Mn5+ complex. Treatment of Mn2+ S49A oxalate oxidase generates the same yellow species as the glycosylated wild type enzyme. Mass spectra of isolated and periodate-treated oxalate oxidase are virtually identical, demonstating that no protein oxidation occurred. Peroxidate oxidation increases the specific activity about 5fold. 674894 1.2.3.4 additional information incubation of the apoenzyme with a 100fold molar excess of MgCl2, CoCl2, CuCl2, ZnCl2, NiCl2, FeCl2 or FeCl3, individually, does not influence enzymatic activity 724114 1.2.3.4 additional information Na2SO4, EDTA-Na2, KCl, NaCl, K2CO3, Na2CO3, Na2HPO4 and NaH2PO4 have no significant effect on the enzyme activities 741123 1.2.3.4 additional information not activated by Mg2+, Co2+, Zn2+, Ni2+, and Fe2+ 726327 1.2.3.4 additional information OXO gene expression is induced by Al3+ 654148 1.2.3.4 additional information the enzymatic activity is not influenced by K+, Na+, Zn2+, Fe3+, Mn2+, and Mg2+ 741317 1.2.3.4 additional information the enzyme is unaffected by 0.1 mM NaCl and Ca2+ 725122 1.2.3.4 additional information the immobilized oxalate oxidase shows no increase in activity in the presence of 10 mM K+ or Na+ 684408 1.2.3.4 NaCl specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NH4Cl 674758 1.2.3.4 NaCl the specific activity of oxalate oxidase is increased in seedlings grown in a NaCl containing medium compared to normal, which reveals the increased de novo synthesis of the enzyme to sustain oxalate egradation 675632 1.2.3.4 NH4Cl specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NaCl 674758 1.2.3.4 SDS enzyme activity of the C isoform is lost in the presence of sodium dodecyl sulfate 671415 1.2.3.4 Zn2+ 0.36 atoms per subunit 288392 1.2.3.4 Zn2+ 0.36-0.62 g per mol protein 288378