1.2.1.B25 Ca2+ activates, inhibitory as it causes precipitation of the acyl-CoA substrate at 5 mM 1.2.1.B25 K+ activates about 10fold 1.2.1.B25 Mg2+ best activating divalent cation 1.2.1.B25 Mn2+ activates, inhibitory as it causes precipitation of the acyl-CoA substrate at 2 mM 1.2.1.B25 additional information ACR requires divalent metal ions for activity. 2-mercaptoethanol and Na+ have no effect on activity