1.17.1.9 Ca2+ 1 mM Ca2+ improves the enzyme activity by 77% 724094 1.17.1.9 Ca2+ the enzyme activity is increased 13.8% over the control by 1 mM Ca2+ 725222 1.17.1.9 Co2+ 1 mM Co2+ improves the enzyme activity by 46% 724094 1.17.1.9 Co2+ the enzyme activity is increased 9.8% over the control by 1 mM Co2+ 725222 1.17.1.9 Fe 5-8 mol of iron per mol of enzyme 288087 1.17.1.9 Fe contains 11-18 gatoms of non-heme iron per mol of enzyme 288083 1.17.1.9 Fe formate dehydrogenase I contains 18-25 mol of iron per mol of enzyme. Formate dehydrogenase II contains 6-10 mol of iron per mol of enzyme 288092 1.17.1.9 Fe two distinct [Fe-S] centres 288120 1.17.1.9 Fe2+ the enzyme activity is increased 49.4% over the control by 1 mM Fe2+ 725222 1.17.1.9 Iron contains 5 mol nonheme iron per mol of enzyme. The beta-subunit contains a putative binding motif for an iron-sulfur cluster. The alpha-subunit contains at least one iron-sulfur cluster 655461 1.17.1.9 K+ the enzyme activity is increased 6.7% over the control by 1 mM K+ 725222 1.17.1.9 Li+ the enzyme activity is increased 13.8% over the control by 1 mM Li+ 725222 1.17.1.9 Mg2+ the enzyme activity is increased 7.6% over the control by 1 mM Mg2+ 725222 1.17.1.9 Mn2+ the enzyme activity is increased 29.8% over the control by 1 mM Mn2+ 725222 1.17.1.9 Molybdenum 0.64 gatom per mol of enzyme. The enzyme contains molybdopterin guanine dinucleotide 288123 1.17.1.9 Molybdenum contains molybdenum 740767, 740775 1.17.1.9 Molybdenum molybdoenzyme 697332 1.17.1.9 additional information Li+, Na+, K+, NH4+, Cl?, HPO42-, HCO3-, and SO42- at 100 mM have scarcely effect on the enzymatic activity. The enzyme activity is very slightly increased in the presence of 1 mM Co2+, Ni2+, Cu2+, Zn2+, Fe3+, Mn2+, Mg2+, Ca2+, and NH4+ 740397 1.17.1.9 additional information Mn2+, Ba2+, and Mg2+ do not significantly change the enzyme activity 724094 1.17.1.9 additional information no divalent metal cation is needed as a cofactor for enzyme activity 686800 1.17.1.9 Tungsten contains 1.8 mol tungsten per mol of enzyme 655461