1.14.13.81 Fe2+ required 1.14.13.81 Fe2+ requirement 1.14.13.81 Fe2+ a ferritin-like, diiron-carboxylate protein, ion ions have a catalytic function in the enzyme 1.14.13.81 Fe2+ required, presence of a diiron cluster in AcsF 1.14.13.81 Fe2+ required, the aerobic cyclase belongs to the family of diiron carboxylate-bridged proteins characterized by the iron-binding motif E-Xn-E-X-X-H-Xn-E-Xn-E-X-X-H 1.14.13.81 FeSO4 when added with 1,10-phenanthroline 1.14.13.81 Mg2+ required