1.1.1.93 Co2+ 1 mM, 30% of the activation with 0.4 mM MnCl2 389609 1.1.1.93 K+ 50 mM K2SO4, 56% of the activation with 50 mM KCl 389609 1.1.1.93 K+ 50 mM KCl, 63% of the activation with 50 mM NH4Cl 389609 1.1.1.93 K+ monovalent cation required, maximal activity with K+ and Rb+ 389612 1.1.1.93 K+ required for binding of meso-tartrate 667741 1.1.1.93 Mg2+ 5 mM, 59% of the activation with 0.4 mM MnCl2 389609 1.1.1.93 Mg2+ activates 389618 1.1.1.93 Mg2+ optimal concentration: 50 mM 389619 1.1.1.93 Mg2+ required 389619 1.1.1.93 Mg2+ supports catalytic activity 389612 1.1.1.93 Mn2+ activates 389618 1.1.1.93 Mn2+ Km: 0.016 mM, reaction with L-malate or D-malate 389609 1.1.1.93 Mn2+ monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM 389609 1.1.1.93 Mn2+ required 389610, 389612 1.1.1.93 Mn2+ required for binding of meso-tartrate 667741 1.1.1.93 Mn2+ supports catalytic activity 389612 1.1.1.93 additional information the reaction requires a divalent metal ion for activity 654622 1.1.1.93 NaCl 50 mM KCl, 19% of the activation with 50 mM NH4Cl 389609 1.1.1.93 Rb+ monovalent cation required, maximal activity with K+ and Rb+ 389612 1.1.1.93 Zn2+ 0.1 mM, 14% of the activation with 0.4 mM MnCl2 389609