4.1.1.112 Mn2+ required, can be substituted by Mg2+, Co2+, Ni2+, Ca2+ 4276 4.1.1.112 Mn2+ the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+ 714979 4.1.1.112 Na+ dependent on 663796 4.1.1.112 Na+ protects against tryptic digestion 651122 4.1.1.112 Ni2+ activates 4272, 664936 4.1.1.112 Zn2+ active site metal ion, 2 mol Zn2+ per mol enzyme 681424 4.1.1.112 Zn2+ binds to the gamma subunit 650185, 651122 4.1.1.112 Zn2+ bound at the gamma-subunit, coordinated by several residues at the hydrophilic C-terminus 726821 4.1.1.112 Zn2+ subunit Oad-gamma contains a Zn(II)-bound metal 715662 4.1.1.112 Zn2+ the gamma subunit contains zinc 716673