2.4.1.149 Ca2+ about one third of the activity compared to Mn2+, recombinant enzyme 658728 2.4.1.149 Ca2+ activates, 48% as effective as Mn2+ 638606 2.4.1.149 Cd2+ CdCl2 slightly stimulates 489473 2.4.1.149 Co2+ activates, 52% as effective as Mn2+ 638606 2.4.1.149 Co2+ slightly stimulates, 12.8% as effective as Mn2+ at 10 mM 638578 2.4.1.149 Mg2+ about one third of the activity compared to Mn2+, recombinant enzyme 658728 2.4.1.149 Mg2+ MgCl2 slightly stimulates 489473 2.4.1.149 Mn2+ essential for activity, neither Mg2+ nor Ca2+ can substitute for Mn2+ 638575 2.4.1.149 Mn2+ MnCl2 stimulates 2.5fold, optimal concentration: 20 mM 638577 2.4.1.149 Mn2+ MnCl2: 10-25 mM for optimal activity required 489473