1.13.11.6 (NH4)2SO4 the most effective salt, the optimal concentration is 0.035 M 439364 1.13.11.6 Fe2+ solvent, acid and heat function to modify the protein configuration so that ferrous ions can be bound to the enzyme to generate the most active form 439364 1.13.11.6 HCl during purification of enzyme treatment with acid was used 439364 1.13.11.6 MgCl2 - 439364 1.13.11.6 NaCl - 439364 1.13.11.6 Fe2+ one Fe2+ per active site 439365 1.13.11.6 Fe3+ does not seem to bind to the enzyme 439365 1.13.11.6 Fe2+ requirement 439366, 439370, 439377 1.13.11.6 Fe2+ needed for catalytic activity 649382 1.13.11.6 Fe each monomer contains two iron binding sites. The catalytic iron is buried deep inside the beta-barrel with His51, Glu57, and His95 serving as ligands. The other iron site forms an FeS4 center close to the solvent surface in which the sulfur atoms are provided by Cys125, Cys128, Cys162, and Cys165. The two iron sites are separated by 24 A 672037 1.13.11.6 Fe2+ non-heme ferrous enzyme 677020 1.13.11.6 Ni2+ two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site 677020 1.13.11.6 Fe2+ required, the active site of HAD contains an iron(II) center that is coordinated by the 2-His-1-Glu facial triad 723950 1.13.11.6 Fe2+ a non-heme iron-containing enzyme, binding structure, overview 741516 1.13.11.6 additional information comparison of the active sites of Fe(III)-h3HAO and Zn(II)-h3HAO enzymes, overview 741516 1.13.11.6 Fe2+ a non-heme iron-containing enzyme 743276 1.13.11.6 Iron enhanced hydrophobicity at the iron center results from the concerted loop movements after the binding of the primary substrate 765043 1.13.11.6 Iron nonheme iron-based enzyme 765681