7.6.2.8 cell membrane transmembrane enzyme - 751956 7.6.2.8 cytoplasm - 5737 667642 7.6.2.8 cytoplasmic membrane btuC and btuD: associated - 210329 7.6.2.8 cytoplasmic membrane btuC: integral - 210326, 210327 7.6.2.8 cytoplasmic membrane btuD: associated - 210326, 210327 7.6.2.8 endoplasmic reticulum membrane translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1 5789 752200 7.6.2.8 inner membrane - - 719509, 750063 7.6.2.8 inner membrane integral membrane enzyme - 751670 7.6.2.8 inner membrane transmembrane enzyme - 751644, 751956 7.6.2.8 lysosomal membrane ABCD4 dimer makes a complex with lysosomal membrane protein LMBD1, an adopter protein, and targets to lysosome 5765 749821 7.6.2.8 lysosomal membrane translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1 5765 752200 7.6.2.8 membrane - 16020 734003, 734820, 751661, 752215 7.6.2.8 membrane integral membrane enzyme 16020 -, 750412 7.6.2.8 membrane periplasmic and cytosolic orientation 16020 697950 7.6.2.8 membrane periplasmic orientation 16020 696776 7.6.2.8 membrane transmembrane enzyme 16020 -, 749978 7.6.2.8 additional information ABCD4 barely interacts with the peroxisomal biogenesis factor Pex19p, because of its lack of NH2-terminal hydrophobic motif. As a result, the transmembrane domain (TMD) 1 of ABCD4 is recognized by certain signal recognition particles and integrated into the ER membrane. ABCD4 dimer then makes a complex with lysosomal membrane protein LMBD1, an adopter protein, and targets to lysosome - 749821 7.6.2.8 additional information LMBD1 associates with ABCD4 at the ER membrane and transports ABCD4 to lysosomes, ABCD4 interacts with LMBD1. The translocation depends on the lysosomal targeting ability of LMBD1. Endogenous ABCD4 is localized to both lysosomes and the endoplasmic reticulum. ABCD4 lacks the NH2-teminal hydrophobic region that is responsible for the targeting to peroxisomes. ABCD4 and LMBD1 form complex in vitro. The regions around transmembrane helix 2 and 5 of ABCD4 are critically important for the translocation of ABCD4 to lysosomes along with LMBD1 - 752200 7.6.2.8 periplasm - - 667642, 685066, 685614, 685626, 690056, 751661 7.6.2.8 periplasm btuE - 210326, 210329