2.5.1.32 amyloplast isoform PSY1 is localized to amyloplast envelope membrane 9501 689617 2.5.1.32 chloroplast - 9507 676344, 689623, 739376, 739712, 760016 2.5.1.32 chloroplast enzyme contains a chloroplast transit peptide 9507 659686 2.5.1.32 chloroplast enzyme contains a putative N-terminal chloroplast transit peptide 9507 659796 2.5.1.32 chloroplast localized at thylakoid membranes in mature chloroplasts 9507 637886 2.5.1.32 chloroplast more highly expressed in chromoplasts compared to chloroplasts 9507 637873 2.5.1.32 chloroplast sequence contains a 51-residue transit peptide 9507 689600 2.5.1.32 chromoplast - 9509 637882 2.5.1.32 chromoplast more highly expressed in chromoplasts compared to chloroplasts 9509 637873 2.5.1.32 chromoplast peripheral plastid membrane 9509 637876 2.5.1.32 chromoplast stroma 9509 637872 2.5.1.32 etioplast prolamellar body under certain light conditions, e.g. far-red light, the increase in PSY mRNA and protein levels are not accompanied by an increase in enzymatic activity. Under those conditions the enzyme is localized in the prolamellar body fraction in a mostly enzymatically inactive form 9541 637886 2.5.1.32 membrane - 16020 676344 2.5.1.32 membrane isoform PSY1 is localized to amyloplast envelope membrane 16020 689617 2.5.1.32 membrane membrane-associated. Treatment with high ionic strength buffer or mild non-ionic detergent is required for solubilization 16020 637885 2.5.1.32 membrane peripheral plastid membrane 16020 637876 2.5.1.32 membrane the soluble enzyme is complexed and inactive, the membrane-bound enzyme is active 16020 637882 2.5.1.32 additional information no predictable plastid transit peptide in the N-terminal region of EgCrtB. The stigma of the alga is surrounded by a single membrane and has no structural similarity to the chloroplast. This suggests that EgCrtB might be transported to stigmata as well as plastids and that EgCrtB might contain an as-yet unidentified signal sequence - -, 737951 2.5.1.32 plastid - 9536 2941, 637870, 637875, 739376, 759668 2.5.1.32 soluble the soluble enzyme is complexed and inactive, the membrane-bound enzyme is active - 637882 2.5.1.32 soluble upon induction the recombinant enzyme constitutes 5-10% of the total soluble protein - 637884