2.3.1.158	chloroplast envelope	-	
2.3.1.158	chloroplast outer membrane	-	
2.3.1.158	endoplasmic reticulum	-	
2.3.1.158	endoplasmic reticulum membrane	-	
2.3.1.158	lipid droplet	-	
2.3.1.158	membrane	-	
2.3.1.158	membrane	soluble form is produced for comparison with the native membrane bound enzyme	
2.3.1.158	microsome	-	
2.3.1.158	additional information	phylogenetic analysis showed that plant PDAT can be grouped into four clades, two of which have one putative transmembrane domain (TMD) while the other two are predicted to be entirely soluble. The majority of PDAT in the database have the single-predicted TMD consisting of a small cytosolic N-terminus and a large C-terminal domain in the endoplasmic reticulum lumen. The N-terminal region is hydrophilic with arginine clusters similar to those observed in DGAT1	
2.3.1.158	additional information	phylogenetic analysis shows that plant PDAT can be grouped into four clades, two of which have one putative transmembrane domain (TMD) while the other two are predicted to be entirely soluble. The majority of PDAT in the database have the single-predicted TMD consisting of a small cytosolic N-terminus and a large C-terminal domain in the endoplasmic reticulum lumen. The N-terminal region is hydrophilic with arginine clusters similar to those observed in DGAT1	
2.3.1.158	plasma membrane	-	
2.3.1.158	soluble	SiPDAT2 does not have any transmembrane domains