4.1.3.39 0.0044 - (S)-4-hydroxy-2-oxopentanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C 724380 4.1.3.39 0.0048 - (S)-4-hydroxy-2-oxohexanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C 724380 4.1.3.39 0.012 - (4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.013 - (4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.013 - (4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.013 - (4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.089 - (4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C 714244 4.1.3.39 0.089 - (S)-4-hydroxy-2-oxopentanoate in the presence of NADH, in 100 mM HEPES pH 8.0, at 25°C 724341 4.1.3.39 0.158 - (S)-4-hydroxy-2-oxopentanoate in the absence of NADH, in 100 mM HEPES pH 8.0, at 25°C 724341 4.1.3.39 0.222 - (4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C 714244