4.1.3.3 additional information - additional information with substrate N-acetylneuraminic acid increasing Km with increasing pH 33315 4.1.3.3 0.013 - N-acetylneuraminate purified recombinant mutant K165C, pH 7.4, 30°C 727317 4.1.3.3 0.015 - N-acetylneuraminate purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C 727317 4.1.3.3 0.023 - N-acetylneuraminate purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C 727317 4.1.3.3 0.036 - N-acetylneuraminate purified recombinant wild-type enzyme, pH 7.4, 30°C 727317 4.1.3.3 0.04 - N-acetylneuraminate purified recombinant wild-type enzyme, pH 6.8, 30°C 727317 4.1.3.3 0.2 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192M mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.2 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192P mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.27 - N-acetylneuraminate mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 0.3 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192F mutant protein, pH 7.5, 25°C 715921