4.1.2.52 additional information - additional information apparent Km values for Co2+ increase in the two mutants H45A and H45Q by about 800fold compared to the wild-type enzyme 722258 4.1.2.52 0.006 - 4-hydroxy-2-oxopentanoate Km (app) with Co2+, pH 8.0, 25°C 721601 4.1.2.52 0.0175 - 4-hydroxy-2-oxopentanoate Km (app) with Mn2+, pH 8.0, 25°C 721601 4.1.2.52 0.05 - 2-oxo-3-methyl-4-hydroxypentanoate wild type enzyme, at pH 8.0 and 25°C 725493 4.1.2.52 0.16 - 4-hydroxy-2-oxohexanoate pH 8.0, 25°C 721601 4.1.2.52 0.289 - 4-hydroxy-2-oxopentanoate Km (app) with Mg2+, pH 8.0, 25°C 721601 4.1.2.52 0.35 - 4-hydroxy-2-oxoheptane-1,7-dioate using H2O as solvent, in the presence of 0.5 mM Co2+, at pH 8.0 and 25°C 725493 4.1.2.52 0.38 - (S)-4-hydroxy-2-oxopentanoate wild type enzyme, with 0.5 mM Co2+, at pH 8.0 and 25°C 725493 4.1.2.52 0.38 - 4-hydroxy-2-oxopentanoate pH 8.0, 25°C 721601 4.1.2.52 0.38 - 4-hydroxy-2-oxopentanoic acid wild-type, pH 8.0, 25°C 722258