1.8.4.9 additional information - additional information thermodynamic parameters for binding of AtAPR1 redox domain to GSH or GSSG 763921 1.8.4.9 0.0002 - glutathione - 667746 1.8.4.9 0.00057 - 5'-adenylylsulfate assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C 667703 1.8.4.9 0.0025 - 5'-adenylylsulfate with glutathione as reductant 288642 1.8.4.9 0.0033 - 5'-adenylylsulfate in the presence of 0.001 mM thioredoxin 669891 1.8.4.9 0.0038 - 5'-adenylylsulfate with DTT as reductant 288642 1.8.4.9 0.0052 - 5'-adenylylsulfate in the presence of 0.01 mM thioredoxin 669891 1.8.4.9 0.0065 - 5'-adenylylsulfate with 10 mM glutathione as reductant 288644 1.8.4.9 0.0065 - 5'-adenylylsulfate with glutathione as reductant 288643 1.8.4.9 0.011 - 5'-adenylyl sulfate - 667746