1.2.4.1 additional information - additional information detailed kinetic and thermodynamic analysis 672086 1.2.4.1 additional information - additional information kinetics 655474 1.2.4.1 additional information - additional information sigmoidal dependence on the concentration of pyruvate, Hill-coefficient: 1.85 348960 1.2.4.1 additional information - additional information with aging of rats the Vmax of the enzyme complex increases by 60%, while the Km decreases by 1.6fold in the same time, enzyme expression level remaines constant, more efficient catabolization of pyruvate due to adaptive phosphorylation of the enzyme within the multienzyme complex, age-related decline in pyruvate dehydrogenase kinase PDK-4 654462 1.2.4.1 0.00056 - thiamine diphosphate mutant R267A, pH 7.0, 25°C 655474 1.2.4.1 0.0009 - thiamine diphosphate recombinant enzyme 348987 1.2.4.1 0.0011 - pyruvate wild-type enzyme, pH 7.0, 25°C 655474 1.2.4.1 0.0011 - pyruvate wild-type, pH 7.0, 30°C 735664 1.2.4.1 0.0012 - pyruvate mutant F266A, pH 7.0, 25°C 655474 1.2.4.1 0.0015 - thiamine diphosphate recombinant enzyme 348987