1.17.1.9 additional information - additional information - 288115 1.17.1.9 additional information - additional information effect of organic solvents of formate dehydrogenase 288091 1.17.1.9 additional information - additional information the enzyme has two sites with about equal catalytic activity. The Km-values for formate are different for the two catalytic sites: 1.67 mM and 6.25 mM 288112 1.17.1.9 0.003 - NADH at pH 6.0 and 35°C 743715 1.17.1.9 0.0033 - formate pH 7.5, 30°C, heated DE-52-fraction 656618 1.17.1.9 0.0057 - NAD+ - 288113 1.17.1.9 0.0086 - NAD+ mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0091 - NAD+ mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0109 - NAD+ mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.011 - formate pH 7.5, 30°C, enzyme expressed in tobacco 656618