1.14.15.8 additional information - additional information KM-value for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 696462 1.14.15.8 0.0007 - progesterone pH 7.4, 30°C, mutant K27R/I71T/I215T 697323 1.14.15.8 0.0009 - progesterone pH 7.4, 30°C, mutant I86T 697323 1.14.15.8 0.001 - progesterone pH 7.4, 30°C, mutant E90V/D185G 697323 1.14.15.8 0.0012 - deoxycorticosterone pH 7.4, 30°C, mutant I86T 697323 1.14.15.8 0.0015 - deoxycorticosterone pH 7.4, 30°C, wild-type enzyme 697323 1.14.15.8 0.0015 - progesterone pH 7.4, 30°C, wild-type enzyme 697323 1.14.15.8 0.0019 - deoxycorticosterone pH 7.4, 30°C, mutant E90V/D185G 697323 1.14.15.8 0.0024 - deoxycorticosterone pH 7.4, 30°C, mutant K27R/I71T/I215T 697323 1.14.15.8 0.0024 - progesterone pH 7.4, 30°C, mutant A395L 697323