6.3.1.8 0.0224 - glutathione mutant enzyme R598A 673330 6.3.1.8 0.052 - ATP pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction 662446 6.3.1.8 0.059 - spermidine pH 7.3, 25°C, synthetase activity 649733 6.3.1.8 0.076 - spermidine mutant enzyme S337A 673330 6.3.1.8 0.076 - spermidine wild type enzyme 673330 6.3.1.8 0.078 - spermidine pH 7.3, 25°C, synthetase activity of the C79A mutant 649733 6.3.1.8 0.096 - MgADP- pH 7.3, 25°C, synthetase activity of the C79A mutant 649733 6.3.1.8 0.114 - MgADP- pH 7.3, 25°C, synthetase activity 649733 6.3.1.8 0.126 - spermidine mutant enzyme S335A 673330 6.3.1.8 0.13 - gamma-L-Glu-L-Cys(S-methyl)-Gly - 966 6.3.1.8 0.153 - spermidine mutant enzyme K607A 673330 6.3.1.8 0.157 - spermidine pH 7.2, 25°C 692316 6.3.1.8 0.171 - glutathione pH 7.3, 25°C, synthetase activity of the C79A mutant 649733 6.3.1.8 0.18 - gamma-Glu-L-Cys(S-ethyl)-Gly - 966 6.3.1.8 0.182 - spermidine mutant enzyme R538A 673330 6.3.1.8 0.215 - ATP pH 7.2, 25°C 692316 6.3.1.8 0.218 - glutathione wild type enzyme 673330 6.3.1.8 0.224 - glutathione mutant enzyme S337A 673330 6.3.1.8 0.242 - glutathione pH 7.3, 25°C, synthetase activity 649733 6.3.1.8 0.251 - glutathione mutant enzyme K607A 673330 6.3.1.8 0.4 - gamma-L-Glu-L-Ile-Gly - 966 6.3.1.8 0.406 - spermidine mutant enzyme C338A 673330 6.3.1.8 0.407 - glutathione mutant enzyme E391A 673330 6.3.1.8 0.5 - glutathionylspermidine pH 7.3, 25°C, amidase activity 649733 6.3.1.8 0.53 - gamma-Glu-L-Cys(S-propyl)-Gly - 966 6.3.1.8 0.609 - glutathione pH 7.2, 25°C 692316 6.3.1.8 0.665 - spermidine mutant enzyme R598A 673330 6.3.1.8 0.67 - gamma-L-Glu-L-Val-Gly - 966 6.3.1.8 0.691 - glutathione mutant enzyme T441A 673330 6.3.1.8 0.794 - glutathione mutant enzyme S335A 673330 6.3.1.8 0.91 - gamma-L-Glu-L-Cys-Gly - 966 6.3.1.8 1.008 - spermidine mutant enzyme E392A 673330 6.3.1.8 1.175 - glutathione pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction 662446 6.3.1.8 1.324 - glutathione mutant enzyme R538A 673330 6.3.1.8 1.61 - spermidine mutant enzyme T441A 673330 6.3.1.8 2.098 - glutathione mutant enzyme C338A 673330 6.3.1.8 4 - gamma-L-Glu-L-Ala-Gly - 966 6.3.1.8 4.522 - spermidine mutant enzyme E391A 673330 6.3.1.8 7.298 - glutathione mutant enzyme E392A 673330 6.3.1.8 7.424 - spermidine pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction 662446 6.3.1.8 14 - gamma-Glu-L-Cys(S-butyl)-Gly - 966