5.4.99.64 0.0075 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) 738677 5.4.99.64 0.008 - (R)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C 738677 5.4.99.64 0.067 - (R)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C 738677 5.4.99.64 0.067 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.087 - (R)-3-hydroxybutanoyl-CoA pH 7.8, 55°C 737504 5.4.99.64 0.298 - (R)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C 738677 5.4.99.64 0.298 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 1.66 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409, 738677 5.4.99.64 1.66 - (R)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C 738677 5.4.99.64 0.0146 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) 738677 5.4.99.64 0.015 - (S)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C 738677 5.4.99.64 0.117 - (S)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C 738677 5.4.99.64 0.117 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.128 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409, 738677 5.4.99.64 0.128 - (S)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C 738677 5.4.99.64 0.313 - (S)-3-hydroxybutanoyl-CoA pH 7.8, 55°C 737504 5.4.99.64 0.433 - (S)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C 738677 5.4.99.64 0.433 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme, I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.663 - (S)-3-hydroxybutanoyl-CoA mutant I90L, pH 6.6, 30°C 738677 5.4.99.64 0.663 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) 738677 5.4.99.64 1.76 - (S)-3-hydroxybutanoyl-CoA mutant I90V, pH 6.6, 30°C 738677 5.4.99.64 1.76 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V 736409 5.4.99.64 1.76 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.0435 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.235 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.374 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme 738677 5.4.99.64 0.977 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.042 - 2-hydroxy-2-methylpropanoyl-CoA mutant D117A, pH 6.6, 30°C 738677 5.4.99.64 0.042 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) 738677 5.4.99.64 0.061 - 2-hydroxy-2-methylpropanoyl-CoA mutant D117V, pH 6.6, 30°C 738677 5.4.99.64 0.0614 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.104 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409, 738677 5.4.99.64 0.104 - 2-hydroxy-2-methylpropanoyl-CoA wild-type, pH 6.6, 30°C 738677 5.4.99.64 0.304 - 2-hydroxy-2-methylpropanoyl-CoA mutant I90A, pH 6.6, 30°C 738677 5.4.99.64 0.304 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.517 - 2-hydroxy-2-methylpropanoyl-CoA mutant I90L, pH 6.6, 30°C 738677 5.4.99.64 0.517 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) 738677 5.4.99.64 1.84 - 2-hydroxy-2-methylpropanoyl-CoA mutant I90V, pH 6.6, 30°C 738677 5.4.99.64 1.84 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V 736409 5.4.99.64 1.84 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.09 - 2-hydroxyisobutanoyl-CoA pH 7.8, 55°C 737504 5.4.99.64 3.34 - butanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409 5.4.99.64 0.55 - isobutanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409 5.4.99.64 0.147 - isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.288 - isovaleryl-CoA pH 6.6, 30°C, wild-type enzyme 738677 5.4.99.64 0.329 - isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677