5.4.99.5 additional information - Chorismic acid Competetive inhibition by I – IV Structur: increase the Km 672622 5.4.99.5 additional information - additional information A lower Km of 0.5 +/-0.05 mM is obtained with a 27.5 nM protein concentration (11 pmol) whereas a Km of 0.67 +/-0.05 nM is obtained with a 8 nM protein concentration (3.2 pmol) 674312 5.4.99.5 additional information - additional information due to instability of chorismate at higher temperature, a Km value is not determined 692260 5.4.99.5 additional information - additional information Michaelis-Menten kinetics 747259, 749355 5.4.99.5 additional information - additional information Michaelis-Menten steady-state kinetics 747032 5.4.99.5 additional information - additional information sigmoid substrate saturation curve with S0.5: 16.7 mM for chorismate at 37°C and S0.5: 12 mM for chorismate at 37°C in presence of 0.1 mM tyrosine 651660 5.4.99.5 additional information - additional information steady-state kinetics, isozyme AtCM1 shows Michaelis-Menten kinetics. Effect of aromatic amino acids on wild-type and mutant AtCM1, overview 748178 5.4.99.5 additional information - additional information steady-state kinetics, isozyme AtCM2, shows Michaelis-Menten kinetics 748178 5.4.99.5 additional information - additional information steady-state kinetics, isozyme AtCM3 displays positive cooperativity with a Hill coefficient of 2.1, indicating that substrate binding at one active site of the homodimer enhanced interaction at the second active site 748178 5.4.99.5 additional information - additional information The Km of the active complementations (position 7, 32, 35, 48, 81 and 85) is shown 672268 5.4.99.5 additional information - additional information when the pH increases from pH 6.2 to pH 8.6 the Km-values for prephenate and chorismate increase substantially 3574 5.4.99.5 0.03 - chorismate mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.04 - chorismate in presence of NAD+ 3557 5.4.99.5 0.041 - chorismate 30°C 649832 5.4.99.5 0.041 - chorismate 30°C, pH 7.2, mutant H347N 390552 5.4.99.5 0.045 - chorismate 30°C, pH 7.2, wild-type 390552 5.4.99.5 0.045 - chorismate in presence of Trp 3554 5.4.99.5 0.051 - chorismate 30°C, pH 7.2, mutant H153N 390552 5.4.99.5 0.053 - chorismate 30°C, pH 7.2, mutant H131A 390552 5.4.99.5 0.054 - chorismate wild type enzyme, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.059 - chorismate mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.067 - chorismate 30°C, pH 7, wild-type 652061 5.4.99.5 0.067 - chorismate pH 7.5, 37°C, recombinant AroH 749355 5.4.99.5 0.068 - chorismate 30°C, pH 7.2, mutant H197N 390552 5.4.99.5 0.073 - chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.074 - chorismate wild-type 651595 5.4.99.5 0.081 - chorismate 30°C, pH 7.5, wild-type, with 4 mM substrate 649938 5.4.99.5 0.092 - chorismate pH 7.5, in absence of NAD+ 3557 5.4.99.5 0.098 - chorismate 30°C, pH 7.2, mutant H257A 390552 5.4.99.5 0.099 - chorismate 30°C, pH 7.2, mutant H265A 390552 5.4.99.5 0.1 - chorismate - 3562 5.4.99.5 0.126 - chorismate 30°C, pH 7.2, mutant H239N 390552 5.4.99.5 0.15 - chorismate 30°C, pH 7, mutant R90G 652061 5.4.99.5 0.15 - chorismate leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 661703 5.4.99.5 0.15 - chorismate recombinant enzyme, pH 8.0, temperature not specified in the publication 748178 5.4.99.5 0.18 - chorismate untagged enzyme, at 30°C and pH 7.5 661703 5.4.99.5 0.185 - chorismate in absence of Trp 3554 5.4.99.5 0.22 - chorismate mutant A32S 663283 5.4.99.5 0.225 - chorismate 30°C, pH 7.2, mutant H245N 390552 5.4.99.5 0.226 - chorismate 37°C, pH 7.8, in presence of P-protein 650528 5.4.99.5 0.24 - chorismate enzyme form CM2 3550 5.4.99.5 0.249 - chorismate mutant L7I 663283 5.4.99.5 0.29 - chorismate 70°C, pH 7.6 649515 5.4.99.5 0.29 - chorismate genetically engineered monofunctional chorismate mutase that contains only 109 amino acids 3560 5.4.99.5 0.296 - chorismate 37°C, pH 7.8 650528 5.4.99.5 0.3 - chorismate wild-type enzyme 3568 5.4.99.5 0.304 - chorismate wild type 663283 5.4.99.5 0.365 - chorismate mutant V35I 663283 5.4.99.5 0.38 - chorismate - 3536 5.4.99.5 0.39 - chorismate chorismate mutase domain of P-protein 651595 5.4.99.5 0.4 - chorismate chorismate, mutant enzyme Thr226Ile, activated by 0.01 mM Trp 3572 5.4.99.5 0.4 - chorismate wild-type enzyme, activated by 0.01 mM Trp 3572 5.4.99.5 0.45 - chorismate mutant enzyme Lys39Asn 3568 5.4.99.5 0.46 - chorismate - 3541 5.4.99.5 0.47 1 prephenate - 3574 5.4.99.5 0.5 - chorismate +/-0.05, substrate chorismate 674312 5.4.99.5 0.5 - chorismate at 37°C and pH 7.5 692314 5.4.99.5 0.5 - chorismate enzyme form CM2 3564 5.4.99.5 0.5 - chorismate pH 7.0, 37°C 747259 5.4.99.5 0.52 - chorismate - 3540, 3555 5.4.99.5 0.53 - chorismate - 3537 5.4.99.5 0.549 - prephenate 37°C, pH 7.8, in presence of P-protein 650528 5.4.99.5 0.55 - chorismate - 3566 5.4.99.5 0.55 - chorismate recombinant enzyme, pH 8.0, temperature not specified in the publication 748178 5.4.99.5 0.57 - chorismate mutant enzyme G86A 703449 5.4.99.5 0.59 - chorismate mutant enzyme Lys39Arg 3568 5.4.99.5 0.59 1 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine 650528 5.4.99.5 0.628 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine 650528 5.4.99.5 0.669 - chorismate mutant I81L/V85I 663283 5.4.99.5 0.7 - chorismate mutant enzyme Tyr234Phe, activated by 0.01 mM Trp 3572 5.4.99.5 0.8 - chorismate mutant enzyme Tyr234Ala, activated by 0.01 mM Trp 3572 5.4.99.5 1 - chorismate strain WB672 3534 5.4.99.5 1.036 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine 650528 5.4.99.5 1.1 - chorismate enzyme CM1 3564 5.4.99.5 1.1 - chorismate recombinant enzyme, pH 8.0, temperature not specified in the publication 748178 5.4.99.5 1.14 - chorismate wild type enzyme 703449 5.4.99.5 1.16 - chorismate mutant enzyme Lys39Gln 3568 5.4.99.5 1.2 - chorismate - 662365 5.4.99.5 1.2 - chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 1.3 - chorismate mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 1.3 - chorismate mutant enzyme Tyr234Ser, activated by 0.01 mM Trp 3572 5.4.99.5 1.5 - chorismate at 37°C and pH 7.5 692314 5.4.99.5 1.5 - chorismate mutant enzyme Ile225Thr/Thr226Ile, activated by 0.01 mM Trp 3572 5.4.99.5 1.514 - chorismate pH 7.5, 37°C, recombinant BsCM_2 749355 5.4.99.5 1.6 - chorismate 37°C, in presence of 0.01 mM tryptophan 651660 5.4.99.5 1.6 - chorismate mutant enzyme Glu23Asp, activated by 0.01 mM Trp 3572 5.4.99.5 1.7 - chorismate enzyme form CM1, weak positive cooperativity 3550 5.4.99.5 1.9 - chorismate 30°C, pH 7, mutant C88K/R90S 652061 5.4.99.5 2.33 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 2.39 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 2.547 - chorismate 37°C, pH 7.8, DELTA102-285 650528 5.4.99.5 2.6 - chorismate strain 168 3534 5.4.99.5 2.83 - chorismate at 37°C 705570 5.4.99.5 3.19 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 3.9 - chorismate 30°C, pH 7.5, mutant DELTA118-127, with 5.8 mM substrate 649938 5.4.99.5 4 - chorismate 30°C, pH 7.5, mutant DELTA119-127/D118N, with 5.8 mM substrate 649938 5.4.99.5 4.1 - chorismate 30°C, pH 7.5, mutant DELTA118-127, with 3.4 mM substrate 649938 5.4.99.5 4.3 - chorismate 30°C, pH 7, mutant C88S/R90K 652061 5.4.99.5 5 - chorismate - 662365 5.4.99.5 5.19 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 5.2 - chorismate mutant enzyme Glu23Ala, activated by 0.01 mM Trp 3572 5.4.99.5 6 - chorismate mutant enzyme Glu23Gln, activated by 0.01 mM Trp 3572 5.4.99.5 6.3 - chorismate mutant enzyme Tyr234Glu, activated by 0.01 mM Trp 3572 5.4.99.5 6.79 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 9.3 - chorismate 30°C, pH 7.5, mutant DELTA117-127, with 3.8 mM substrate 649938 5.4.99.5 9.6 - chorismate DELTA 117-127 651595 5.4.99.5 15 - chorismate 30°C, pH 7.5, mutant DELTA118-127/K111N/A112S/V113N, with 4 mM substrate 649938 5.4.99.5 16 - chorismate 30°C, pH 7.5, mutant DELTA118-127/R116L/P117T, with 3.9 mM substrate 649938