5.3.3.2 additional information - additional information substrate binds to the inactive oxidized and active reduced form of enzyme with similar affinities 678278 5.3.3.2 0.0008 - isopentenyl diphosphate mutant enzyme W161F 650979 5.3.3.2 0.001 - isopentenyl diphosphate isomerase II 2952 5.3.3.2 0.0013 - isopentenyl diphosphate isomerase II and III 2937 5.3.3.2 0.0015 - isopentenyl diphosphate pH 6.0, 60°C, mutant enzyme Q160L 728667 5.3.3.2 0.00152 - isopentenyl diphosphate mutant enzyme Q160L, in 50 mM MOPS-NaOH, pH 6.0, at 60°C 728667 5.3.3.2 0.0017 - isopentenyl diphosphate isomerase I 2937 5.3.3.2 0.0025 - isopentenyl diphosphate isomerase IV 2937 5.3.3.2 0.0027 - isopentenyl diphosphate - 2936 5.3.3.2 0.003 - isopentenyl diphosphate mutant enzyme R83K 650979 5.3.3.2 0.0032 - isopentenyl diphosphate pH 6.0, 60°C, mutant enzyme Q160N 728667 5.3.3.2 0.00323 - isopentenyl diphosphate mutant enzyme Q160N, in 50 mM MOPS-NaOH, pH 6.0, at 60°C 728667 5.3.3.2 0.0033 - isopentenyl diphosphate wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C 727623 5.3.3.2 0.0035 - isopentenyl diphosphate wild-type enzyme 650979 5.3.3.2 0.004 - isopentenyl diphosphate - 2935 5.3.3.2 0.0048 - isopentenyl diphosphate aerobic, presence of NADPH, pH 7.0, 37°C 678309 5.3.3.2 0.0051 - isopentenyl diphosphate isomerase I 2952 5.3.3.2 0.0054 - isopentenyl diphosphate - 2944 5.3.3.2 0.0056 - isopentenyl diphosphate pH 7.0, 37°C 678278 5.3.3.2 0.0057 - isopentenyl diphosphate - 2938, 2941 5.3.3.2 0.00595 - isopentenyl diphosphate wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C 747106 5.3.3.2 0.006 - isopentenyl diphosphate - 2945 5.3.3.2 0.006 - isopentenyl diphosphate mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C 727623 5.3.3.2 0.006 - isopentenyl diphosphate mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C 727623 5.3.3.2 0.0071 - isopentenyl diphosphate pH 6.0, 60°C, mutant enzyme Q160H 728667 5.3.3.2 0.00713 - isopentenyl diphosphate mutant enzyme Q160H, in 50 mM MOPS-NaOH, pH 6.0, at 60°C 728667 5.3.3.2 0.00714 - isopentenyl diphosphate pH 6.0, 60°C, mutant enzyme E13R/R235E 719740 5.3.3.2 0.00739 - isopentenyl diphosphate wild type enzyme, in 50 mM MOPS-NaOH, pH 6.0, at 60°C 728667 5.3.3.2 0.0074 - isopentenyl diphosphate pH 6.0, 60°C, wild-type enzyme 728667 5.3.3.2 0.0076 - isopentenyl diphosphate - 2946 5.3.3.2 0.00826 - isopentenyl diphosphate mutant enzyme Q160E, in 50 mM MOPS-NaOH, pH 6.0, at 60°C 728667 5.3.3.2 0.0083 - isopentenyl diphosphate pH 6.0, 60°C, mutant enzyme Q160E 728667 5.3.3.2 0.0095 - isopentenyl diphosphate wild-type, pH 7.4, 37°C 680660 5.3.3.2 0.0115 - isopentenyl diphosphate mutant enzyme E87Q 650979 5.3.3.2 0.0116 - isopentenyl diphosphate mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C 727623 5.3.3.2 0.012 - isopentenyl diphosphate aerobic, presence of dithionite, pH 7.0, 37°C 678309 5.3.3.2 0.0128 - isopentenyl diphosphate mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C 727623 5.3.3.2 0.014 - isopentenyl diphosphate mutant enzyme K55A 650979 5.3.3.2 0.0142 - isopentenyl diphosphate mutant Y104F, pH 7.4, 37°C 680660 5.3.3.2 0.015 - isopentenyl diphosphate mutant enzyme K55R 650979 5.3.3.2 0.0168 - isopentenyl diphosphate anaerobic, presence of NADPH, pH 7.0, 37°C 678309 5.3.3.2 0.017 - dimethylallyl diphosphate isomerase I 2952 5.3.3.2 0.0185 - isopentenyl diphosphate mutant enzyme R51K 650979 5.3.3.2 0.02 - isopentenyl diphosphate - 2942 5.3.3.2 0.021 - isopentenyl diphosphate mutant enzyme E116Q 650979 5.3.3.2 0.0225 - isopentenyl diphosphate mutant Y104A, pH 7.4, 37°C 680660 5.3.3.2 0.0228 - isopentenyl diphosphate pH 8.0 680882 5.3.3.2 0.033 - isopentenyl diphosphate - 2948 5.3.3.2 0.035 - isopentenyl diphosphate - 2939 5.3.3.2 0.036 - isopentenyl diphosphate - 2932 5.3.3.2 0.0376 - isopentenyl diphosphate mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C 748596 5.3.3.2 0.04 - isopentenyl diphosphate at pH 7.0 and 37°C 747530 5.3.3.2 0.0408 - isopentenyl diphosphate wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C 747106 5.3.3.2 0.0415 - isopentenyl diphosphate wild type enzyme, at pH 7.5 and 30°C 748596 5.3.3.2 0.043 - isopentenyl diphosphate - 2957 5.3.3.2 0.046 - isopentenyl diphosphate pH 8.0, 50°C 660902 5.3.3.2 0.0463 - isopentenyl diphosphate pH 6.0, 60°C, wild-type enzyme 719740 5.3.3.2 0.047 - isopentenyl diphosphate pH 8.0, 55°C 660902 5.3.3.2 0.063 - isopentenyl diphosphate pH 6.0, 60°C 651170 5.3.3.2 0.063 - isopentenyl diphosphate pH 8.0, 60°C 660902 5.3.3.2 0.064 - isopentenyl diphosphate - 651733 5.3.3.2 0.065 - isopentenyl diphosphate pH 8.0, 70°C 660902 5.3.3.2 0.071 - isopentenyl diphosphate pH 8.0, 65°C 660902 5.3.3.2 0.077 - isopentenyl diphosphate pH 8.0, 75°C 660902 5.3.3.2 0.084 - isopentenyl diphosphate pH 8.0, 80°C 660902 5.3.3.2 0.0841 - isopentenyl diphosphate pH 6.0 660882 5.3.3.2 0.0874 - NADH dehydrogenase reaction without isopentenyl diphosphate 651170 5.3.3.2 0.0904 - NADH isomerase reaction 651170 5.3.3.2 0.093 - isopentenyl diphosphate pH 8.0, 90°C 660902 5.3.3.2 0.187 - isopentenyl diphosphate pH 8.0, 82.5°C 660902 5.3.3.2 0.199 - isopentenyl diphosphate pH 8.0, 87.5°C 660902 5.3.3.2 0.211 - isopentenyl diphosphate pH 8.0, 85°C 660902 5.3.3.2 0.2768 - isopentenyl diphosphate in 0.4 M Tris, 1 mM dithiothreitol, 10 mM MgCl2, pH 8.0, at 50°C 727624 5.3.3.2 0.572 - isopentenyl diphosphate mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C 747106 5.3.3.2 15.3 - isopentenyl diphosphate pH 7.0, 85°C 678586