5.3.2.6 additional information - additional information 4-OT exhibits hyperbolic kinetics and shows anticooperativity during catalysis 718840 5.3.2.6 additional information - additional information kinetic modeling, overview 719633 5.3.2.6 additional information - additional information steady-state kinetics of wild-type and mutant 4-OTs for trans-3-chloroacrylic acid dehalogenase activity, pH 8.2, 23°C, overview 718844 5.3.2.6 additional information - additional information steady-state kinetics, overview 718830 5.3.2.6 0.017 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A 718874 5.3.2.6 0.017 - 2-hydroxymuconate mutant 4-OT L8R, pH 7.3, 23°C 718844 5.3.2.6 0.027 - 2-hydroxymuconate mutant 4-OT L8R/I52E, pH 7.3, 23°C 718844 5.3.2.6 0.05 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate at pH 7.3 and 22°C 747782 5.3.2.6 0.06 - 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 0.062 - 2-hydroxymuconate mutant 4-OT I52E, pH 7.3, 23°C 718844 5.3.2.6 0.063 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A 718874 5.3.2.6 0.069 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A 718874 5.3.2.6 0.07 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant wild-type hh4-OT 718874 5.3.2.6 0.09 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C 721792 5.3.2.6 0.09 - 2-oxo-4(E)-hexenedioate D-4OT enantiomer, pH 7.3, 30°C 719636 5.3.2.6 0.09 - phenylenolpyruvate pH 7.3, 23°C 721792 5.3.2.6 0.103 - 2-oxo-4(E)-hexenedioate L-4OT enantiomer, pH 7.3, 30°C 719636 5.3.2.6 0.105 - 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 0.11 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate 721792 5.3.2.6 0.11 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 721792 5.3.2.6 0.121 - phenylenolpyruvate pH 7.3, 24°C, recombinant wild-type hh4-OT 718874 5.3.2.6 0.135 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 721792 5.3.2.6 0.135 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A 718874 5.3.2.6 0.14 - 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 0.143 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A 718874 5.3.2.6 0.145 - 2-hydroxymuconate pH 7.3, 30°C 719633 5.3.2.6 0.152 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A 718874 5.3.2.6 0.159 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A 718874 5.3.2.6 0.17 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate 721792 5.3.2.6 0.18 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, wild-type enzyme 718830 5.3.2.6 0.18 - 2-hydroxymuconate mutant enzyme P1A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 0.18 - 2-hydroxymuconate wild-type 4-OT, pH 7.3, 23°C 718844 5.3.2.6 0.189 - 2-oxo-3-hexenedioate pH 7.3, 30°C 719633 5.3.2.6 0.19 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C 721792 5.3.2.6 0.198 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A 718874 5.3.2.6 0.211 - 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 0.23 - phenylenolpyruvate pH 7.3, 23°C 721792 5.3.2.6 0.29 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R11A/R39A 718830 5.3.2.6 0.29 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R39A 718830 5.3.2.6 0.334 - (2E)-2-hydroxy-3-phenylprop-2-enoate wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 0.345 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A 718874 5.3.2.6 0.44 - 2-hydroxymuconate mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 0.47 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R39Q 718830 5.3.2.6 0.512 - 2-hydroxymuconate wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 0.78 - 2-hydroxymuconate mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 1.033 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A 718874 5.3.2.6 1.05 - 2-hydroxymuconate mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 1.11 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C, mutant R11A 718830 5.3.2.6 1.11 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C, wild-type enzyme 718830 5.3.2.6 1.6 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R11A 718830