5.1.1.1 additional information - additional information effect of NaCl on Km-value 650876 5.1.1.1 additional information - additional information enzyme kinetics analysis 747001 5.1.1.1 additional information - additional information Km values of L-Ala in the presence of urea at various concentrations 2033 5.1.1.1 additional information - additional information Michaelis-Menten kinetics 746615, 748310 5.1.1.1 additional information - additional information Michaelis-Menten steady-state kinetics 759787 5.1.1.1 additional information - additional information Vmax 110 micromol/min/mg D-alanine 673766 5.1.1.1 additional information - additional information Vmax 306 U/mg: D-alanine to L-alanine 674974 5.1.1.1 additional information - additional information Vmax 323 micromol/min/mg L-alanine 673766 5.1.1.1 additional information - additional information Vmax 345 U/mg: L-alanine to D-alanine 674974 5.1.1.1 additional information - additional information Vmax for the racemization (D- to L-alanine and L- to D-alanine) is 87.0 and 84.8 U/mg, respectively. 672806 5.1.1.1 0.046 - L-alanine pH 7.5, 37°C, recombinant mutant K39A 748218 5.1.1.1 0.064 - L-alanine pH 7.5, 37°C, recombinant wild-type enzyme 748218 5.1.1.1 0.25 - D-alanine pH 8.2, 37°C 661002 5.1.1.1 0.29 - L-alanine pH 8.2, 37°C 661002 5.1.1.1 0.304 - D-alanine +/-0.034, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.311 - D-alanine +/-0.008, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.38 - L-alanine recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer 759787 5.1.1.1 0.4 - D-Ala - 651364 5.1.1.1 0.4 - D-alanine pH 8.2, 37°C 661002 5.1.1.1 0.4 - L-alanine pH 8.2, 37°C 661002 5.1.1.1 0.402 - D-alanine +/-0.055, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.439 - D-alanine +/-0.080, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.46 - D-alanine pH and temperature not specified in the publication 748310 5.1.1.1 0.48 - D-alanine pH and temperature not specified in the publication 748310 5.1.1.1 0.5 - D-Ala alr gene encoded 2019 5.1.1.1 0.5 - L-Ala - 651364 5.1.1.1 0.513 - D-alanine +/-0.084, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.528 - D-alanine +/-0.079, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.56 - D-alanine pH not specified in the publication, 30°C 746637 5.1.1.1 0.592 - D-alanine +/-0.085, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.604 - D-alanine +/-0.070, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.615 - D-alanine +/-0.032, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 0.7 - D-alanine pH 8.2, 25°C 662288 5.1.1.1 0.7 - L-alanine pH 8.2, 25°C 662288 5.1.1.1 0.89 - D-alanine pH not specified in the publication, 30°C, recombinant enzyme 726584 5.1.1.1 0.97 - L-alanine pH and temperature not specified in the publication 748310 5.1.1.1 1.008 - D-alanine +/-0.069, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 1.049 - L-alanine +/-0.131, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 1.1 - D-Ala 23°C 651364 5.1.1.1 1.1 - D-Ala 23°C, enzyme Alr 650917 5.1.1.1 1.1 - L-Ala 23°C, enzyme Alr 650917 5.1.1.1 1.2 - L-Ala 23°C 651364 5.1.1.1 1.4 - D-Ala 23°C, enzyme DadX 650917 5.1.1.1 1.4 - D-alanine pH 7.6, 37°C, recombinant enzyme 726814 5.1.1.1 1.4 - L-Ala 23°C, enzyme DadX 650917 5.1.1.1 1.401 - L-alanine +/-0.209, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 1.516 - L-alanine +/-0.083, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 1.562 - L-alanine +/-0.256, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 1.7 - L-Ala alr gene encoded 2019 5.1.1.1 1.8 - D-Ala 90°C, pH 9.5 651822 5.1.1.1 1.993 - L-alanine +/-0.269, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 2 - D-Ala - 2015, 2035 5.1.1.1 2.057 - L-alanine +/-0.038, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 2.1 - D-Ala - 2013 5.1.1.1 2.1 - D-alanine at 23°C 672806 5.1.1.1 2.2 - D-Ala - 2014 5.1.1.1 2.2 - D-Ala D-Ala, dadB encoded enzyme 2019 5.1.1.1 2.3 - D-alanine pH 8.0, 30°C, recombinant mutant Y354W 727795 5.1.1.1 2.4 - D-Ala 37°C, pH 9.0 651680 5.1.1.1 2.6 - D-alanine pH 8.0, 30°C, recombinant mutant I222T 727795 5.1.1.1 2.7 - L-Ala - 2015, 2019 5.1.1.1 2.7 - L-Ala D-Ala 2016, 2019 5.1.1.1 2.77 - L-alanine pH not specified in the publication, 30°C, recombinant enzyme 726584 5.1.1.1 2.8 - D-alanine pH 8.0, 30°C, recombinant wild-type enzyme 727795 5.1.1.1 2.8 - L-alanine - 702930 5.1.1.1 3 - L-alanine - 702868 5.1.1.1 3.03 - L-alanine +/-0.114, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 3.603 - L-alanine +/-0.180, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm 695001 5.1.1.1 3.7 - L-alanine pH 7.6, 37°C 746766 5.1.1.1 3.8 - D-Ala pH 9.0 650600 5.1.1.1 3.8 - L-alanine recombinant His-tagged enzyme, pH 8.0, 37°C 759787 5.1.1.1 4 - D-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W 727795 5.1.1.1 4.1 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 4.2 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 4.2 - L-alanine recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer 759787 5.1.1.1 4.25 - L-Ala - 2016 5.1.1.1 4.4 - L-Ala - 2019 5.1.1.1 4.5 - L-alanine pH 8.0, 30°C, recombinant mutant Y354W 727795 5.1.1.1 4.7 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 4.7 - D-alanine pH 7.4, 30°C 662478 5.1.1.1 4.7 - L-alanine pH 8.0, 30°C, recombinant mutant I222T 727795 5.1.1.1 4.8 - L-alanine pH 7.6, 37°C, recombinant enzyme 726814 5.1.1.1 5 - L-Ala 37°C, pH 9.0 651680 5.1.1.1 5.01 - L-alanine - 674974 5.1.1.1 5.1 - L-alanine pH 8.0, 30°C, recombinant wild-type enzyme 727795 5.1.1.1 5.24 - D-alanine - 674974 5.1.1.1 5.4 - D-alanine pH 8.5, temperature not specified in the publication, recombinant mutant K271T 746615 5.1.1.1 5.6 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 5.9 - L-alanine pH 7.6, 37°C 746766 5.1.1.1 6.1 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 6.2 - D-alanine Vmax 37.9 micromol/min/mg 675750 5.1.1.1 6.8 - L-alanine pH and temperature not specified in the publication 748310 5.1.1.1 6.9 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 7 - D-alanine pH 8.5, temperature not specified in the publication, recombinant wild-type enzyme 746615 5.1.1.1 7.3 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 7.4 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 7.8 - L-Ala - 2014 5.1.1.1 7.9 - L-Ala pH 9.0 650600 5.1.1.1 8.1 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 8.1 - L-alanine pH 7.4, 30°C 662478 5.1.1.1 8.2 - L-Ala - 2013 5.1.1.1 8.2 - L-alanine recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer 759787 5.1.1.1 8.3 - L-alanine pH 8.0, 30°C, recombinant mutant I222T/Y354W 727795 5.1.1.1 8.5 - Ala - 2009 5.1.1.1 8.7 - D-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 8.7 - D-alanine pH 7.4, 30°C 662478 5.1.1.1 9.3 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 9.8 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 10 - D-Ala pH 8.3, 30°C 649578 5.1.1.1 10 - D-serine pH 8.2, 37°C 661002 5.1.1.1 10.34 - L-alanine at pH 9.0 and 37°C 717010 5.1.1.1 11 - L-Ala dadB gene encoded 2019 5.1.1.1 12 - D-alanine Vmax = 0.44 mol/s/kg 676382 5.1.1.1 12 - L-alanine recombinant MBP-tagged enzyme, pH 8.0, 37°C 759787 5.1.1.1 12.2 - D-Ala 30°C, pH 8.3 649541 5.1.1.1 12.8 - D-Ala - 2027 5.1.1.1 13.8 - L-alanine pH 8.5, temperature not specified in the publication, recombinant mutant K271T 746615 5.1.1.1 14.91 - D-alanine - 704736 5.1.1.1 15.36 - D-alanine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication 748617 5.1.1.1 16.5 - D-alanine pH 9.5 661808 5.1.1.1 17.4 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 17.6 - L-alanine pH 8.5, temperature not specified in the publication, recombinant wild-type enzyme 746615 5.1.1.1 17.9 - L-Ala 30°C, pH 8.3 649541 5.1.1.1 18 - D-Lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W 727795 5.1.1.1 18.4 - L-alanine in 50 mM potassium phosphate buffer pH 7.4, at 30°C 715408 5.1.1.1 18.4 - L-alanine pH 7.4, 30°C 662478 5.1.1.1 18.9 - L-Ala - 2027 5.1.1.1 20 - L-Ala pH 8.3, 30°C 649578 5.1.1.1 20.16 - D-alanine pH 11.0, 70°C, recombinant enzyme 727440 5.1.1.1 20.4 - D-alanine HPLC analysis 673766 5.1.1.1 20.78 - D-alanine pH 10.5, 40°C, recombinant wild-type enzyme 747001 5.1.1.1 24 - D-Ala - 652947 5.1.1.1 27 - L-serine pH 8.2, 37°C 661002 5.1.1.1 29.4 - L-alanine pH 9.5 661808 5.1.1.1 29.6 - L-alanine Vmax = 1.02 mol/s/kg 676382 5.1.1.1 32 - L-lysine pH 8.0, 30°C, recombinant mutant I222T/Y354W 727795 5.1.1.1 35.9 - L-alanine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication 748617 5.1.1.1 41.79 - L-alanine - 704736 5.1.1.1 43 - L-alanine HPLC analysis 673766 5.1.1.1 50 - L-Ala - 652947 5.1.1.1 52 - D-alanine pH 10.0, 65°C, recombinant mutant Q360Y 749061 5.1.1.1 56.17 - L-alanine pH 10.5, 40°C, recombinant wild-type enzyme 747001 5.1.1.1 58.5 - D-alanine pH 10.0, 65°C, recombinant mutant S173D/Q360Y 749061 5.1.1.1 73.5 - D-Ala 30°C, pH 8.5 650876 5.1.1.1 83.1 - L-alanine pH 10.0, 65°C, recombinant mutant S173D/Q360Y 749061 5.1.1.1 87.8 - L-alanine pH 10.0, 65°C, recombinant mutant Q360Y 749061 5.1.1.1 100 - L-alanine Vmax 909 micromol/min/mg 675750 5.1.1.1 110.3 - D-alanine pH 10.0, 65°C, recombinant mutant Q360I 749061 5.1.1.1 119 - D-Ala - 2034 5.1.1.1 136 - L-alanine pH 10.0, 65°C, recombinant mutant Q360I 749061 5.1.1.1 157.5 - L-alanine pH 10.0, 65°C, recombinant mutant S173D 749061 5.1.1.1 160.9 - D-alanine pH 10.0, 65°C, recombinant mutant S173D 749061 5.1.1.1 167 - L-Ala pH 8.5, 37°C 655267 5.1.1.1 171 - L-Ala 30°C, pH 8.5 650876 5.1.1.1 176.1 - L-alanine pH 10.0, 65°C, recombinant mutant Q360W 749061 5.1.1.1 179 - D-Ala pH 8.5, 37°C 655267 5.1.1.1 216.1 - D-alanine pH 10.0, 65°C, recombinant mutant Q360W 749061 5.1.1.1 381.2 - D-alanine pH 10.0, 65°C, recombinant wild-type enzyme 749061 5.1.1.1 780.2 - L-alanine pH 10.0, 65°C, recombinant wild-type enzyme 749061