4.3.1.B2	additional information	-	additional information	kinetic model	727051	
4.3.1.B2	0.00027	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A	726941	
4.3.1.B2	0.0013	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A	726941	
4.3.1.B2	0.0015	-	L-glutamine	pH 8.0, 30°C, IGP synthase holoenzyme	726928	
4.3.1.B2	0.0015	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-monophosphate	pH 8.5, 25°C	748148	
4.3.1.B2	0.0016	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K	726939	
4.3.1.B2	0.0018	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A	726939	
4.3.1.B2	0.002	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R	726939	
4.3.1.B2	0.002	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 25°C, cosubstrate: L-glutamine, wild-type enzyme	728756	
4.3.1.B2	0.0023	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R	726939	
4.3.1.B2	0.0028	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 25°C, cosubstrate: L-glutamine, HisH/HisF holoenzyme with a T78M mutant subunit HisF	728756	
4.3.1.B2	0.003	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A	726939	
4.3.1.B2	0.0036	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 25°C, cosubstrate: NH4+, wild-type enzyme	728756	
4.3.1.B2	0.0036	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A	726941	
4.3.1.B2	0.004	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C	728731	
4.3.1.B2	0.005	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme	726939	
4.3.1.B2	0.005	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme	726941	
4.3.1.B2	0.0055	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A	726941	
4.3.1.B2	0.0059	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 25°C, cosubstrate: NH4+, HisH/HisF holoenzyme with a T78M mutant subunit HisF	728756	
4.3.1.B2	0.008	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H	726939	
4.3.1.B2	0.0125	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF	727736	
4.3.1.B2	0.0141	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A	726941	
4.3.1.B2	0.0175	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF	727736	
4.3.1.B2	0.021	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, HisF subunit, saturating concentrations of ammonium chloride can not be achieved due to inhibition of the enzyme activity at chloride ion concentrations	726928	
4.3.1.B2	0.0218	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF	727736	
4.3.1.B2	0.026	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A	726941	
4.3.1.B2	0.03	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A	726941	
4.3.1.B2	0.034	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A	726941	
4.3.1.B2	0.0426	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF	727736	
4.3.1.B2	0.046	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R	726939	
4.3.1.B2	0.053	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A	726939	
4.3.1.B2	0.055	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme	726939	
4.3.1.B2	0.055	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme	726941	
4.3.1.B2	0.065	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A	726939	
4.3.1.B2	0.071	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A	726941	
4.3.1.B2	0.072	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R	726939	
4.3.1.B2	0.075	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H	726939	
4.3.1.B2	0.08	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K	726939	
4.3.1.B2	0.098	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A	726939	
4.3.1.B2	0.109	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A	726941	
4.3.1.B2	0.139	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A	726939	
4.3.1.B2	0.24	-	N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate	pH 8.0, 30°C, IGP synthase holoenzyme	726928	
4.3.1.B2	0.32	-	L-glutamine	pH 8.5, 25°C	748148	
4.3.1.B2	0.49	-	L-glutamine	pH 8.0, 30°C, mutant enzyme K196A	726941	
4.3.1.B2	1	1.2	NH4+	pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF	727736	
4.3.1.B2	1	4	L-glutamine	pH 8.0, 30°C, mutant enzyme D359A	726941	
4.3.1.B2	1	4.5	L-glutamine	pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF	727736	
4.3.1.B2	1.3	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K	726939	
4.3.1.B2	1.7	-	L-glutamine	pH 7.0, 30°C	728731	
4.3.1.B2	1.8	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme	726939	
4.3.1.B2	1.8	-	L-glutamine	pH 8.0, 30°C, wild-type enzyme	726941	
4.3.1.B2	1.9	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R	726939	
4.3.1.B2	1.9	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R	726939	
4.3.1.B2	1.96	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A	726939	
4.3.1.B2	2.1	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H	726939	
4.3.1.B2	2.3	-	L-glutamine	pH 8.0, 30°C, mutant enzyme K196A/D359A	726941	
4.3.1.B2	4.8	-	L-glutamine	pH 8.0, 30°C, mutant enzyme N13A	726941	
4.3.1.B2	5.4	-	L-glutamine	pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF	727736	
4.3.1.B2	6.5	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A	726939	
4.3.1.B2	6.5	-	L-glutamine	pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A	726939	
4.3.1.B2	7	-	L-glutamine	pH 8.0, 30°C, mutant enzyme Q397A	726941	
4.3.1.B2	7.4	-	NH4+	pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF	727736	
4.3.1.B2	13.4	-	NH4+	pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF	727736	
4.3.1.B2	14.3	-	NH4+	pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF	727736	
4.3.1.B2	15	-	NH3	pH 8.0, 30°C, HisF subunit	726928	
4.3.1.B2	16	-	NH3	pH 8.0, 30°C, IGP synthase holoenzyme	726928	
4.3.1.B2	266	-	NH4+	pH 8.0, 30°C, HisF subunit	726928	
4.3.1.B2	291	-	NH4+	pH 8.0, 30°C, IGP synthase holoenzyme	726928