4.1.99.5 additional information - additional information complex kinetics of formation of the reaction intermediate, overview 727690 4.1.99.5 additional information - additional information Michaelis-Menten kinetics 749051 4.1.99.5 additional information - additional information the rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction 747074 4.1.99.5 0.035 - octadecanal pH 7, 30°C 648391 4.1.99.5 0.065 - octadecanal 25°C 648393 4.1.99.5 0.069 - n-nonanal pH 7.2, 37°C, recombinant wild-type enzyme 747351 4.1.99.5 0.14 - n-nonanal pH 7.2, 37°C, recombinant mutant A121F 747351 4.1.99.5 0.18 - n-octanal pH 7.2, 37°C, recombinant wild-type enzyme and mutant A121F 747351 4.1.99.5 0.18 - octadecanal pH 7, 37°C 648392 4.1.99.5 0.2 - n-Heptanal recombinant mutant F150Y, pH and temperature not specified in the publication 747409 4.1.99.5 0.244 - n-Heptanal pH 7.2, 22°C, recombinant enzyme with Fd/FNR system 727215 4.1.99.5 0.3 - n-Heptanal recombinant wild-type enzyme, pH and temperature not specified in the publication 747409 4.1.99.5 0.32 - n-Heptanal recombinant mutant L148R, pH and temperature not specified in the publication 747409 4.1.99.5 0.33 - n-Heptanal recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication 747409 4.1.99.5 0.34 - n-Heptanal recombinant mutant L146T, pH and temperature not specified in the publication 747409 4.1.99.5 0.34 - n-Heptanal recombinant mutant W178R, pH and temperature not specified in the publication 747409 4.1.99.5 0.35 - n-Heptanal recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication 747409 4.1.99.5 0.35 - n-Heptanal recombinant wild-type enzyme, pH and temperature not specified in the publication 747409 4.1.99.5 0.38 - n-Heptanal recombinant mutant D49H/N123H, pH and temperature not specified in the publication 747409 4.1.99.5 0.56 - n-Heptanal pH 7.2, 37°C, recombinant mutant A121F 747351 4.1.99.5 0.59 - n-Heptanal pH 7.2, 37°C, recombinant wild-type enzyme 747351 4.1.99.5 0.93 - n-hexanal pH 7.2, 37°C, recombinant wild-type enzyme 747351 4.1.99.5 0.96 - n-hexanal pH 7.2, 37°C, recombinant mutant A121F 747351