4.1.3.39 0.0044 - (S)-4-hydroxy-2-oxopentanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C 724380 4.1.3.39 0.0048 - (S)-4-hydroxy-2-oxohexanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C 724380 4.1.3.39 0.012 - (4R)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.013 - (4R)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.013 - (4S)-4-hydroxy-2-oxopentanoate Y290F mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.013 - (4S)-4-hydroxy-2-oxopentanoate Y290S mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.089 - (4S)-4-hydroxy-2-oxopentanoate wild type protein, pH 8.0, 25°C 714244 4.1.3.39 0.089 - (S)-4-hydroxy-2-oxopentanoate in the presence of NADH, in 100 mM HEPES pH 8.0, at 25°C 724341 4.1.3.39 0.158 - (S)-4-hydroxy-2-oxopentanoate in the absence of NADH, in 100 mM HEPES pH 8.0, at 25°C 724341 4.1.3.39 0.222 - (4S)-4-hydroxy-2-oxopentanoate H20A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 0.228 - (4S)-4-hydroxy-2-oxopentanoate H20S mutant protein, pH 8.0, 25°C 714244 4.1.3.39 11 - pyruvate aldol addition reaction with propionaldehyde, L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 13 - pyruvate aldol addition reaction with propionaldehyde, wild type protein, pH 8.0, 25°C 714244 4.1.3.39 13.4 - Butyraldehyde pH 8.0, 25°C 702377 4.1.3.39 16.8 - propionaldehyde L87A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 20.2 - pyruvate aldol addition reaction with propionaldehyde, L87A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 24.8 - pentaldehyde L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 32.9 - propionaldehyde pH 8.0, 25°C 702377 4.1.3.39 33.3 - glycolaldehyde pH 8.0, 25°C 702377 4.1.3.39 33.3 - pentaldehyde L87A/L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 40.2 - propionaldehyde L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 49.2 - Butyraldehyde L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 62.9 - acetaldehyde pH 8.0, 25°C 702377 4.1.3.39 64.2 - acetaldehyde wild type protein, pH 8.0, 25°C 714244 4.1.3.39 64.28 - acetaldehyde at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate 702377 4.1.3.39 64.8 - acetaldehyde pH 8.0, 25°C 702377 4.1.3.39 73.8 - Succinic semialdehyde pH 8.0, 25°C 702377 4.1.3.39 80.5 - acetaldehyde L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 88.6 - (R,S)-glyceraldehyde pH 8.0, 25°C 702377 4.1.3.39 124.6 - glycolaldehyde at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate 702377 4.1.3.39 124.6 - glycolaldehyde pH 8.0, 25°C 702377 4.1.3.39 135.9 - propionaldehyde at 25°C with 120 microg of BphI, 2 mM MnCl2 and 100 mM pyruvate 702377 4.1.3.39 135.9 - propionaldehyde pH 8.0, 25°C 702377 4.1.3.39 136 - propionaldehyde wild type protein, pH 8.0, 25°C 714244 4.1.3.39 289 - Butyraldehyde L87A/L89A mutant protein, pH 8.0, 25°C 714244 4.1.3.39 323 - Butyraldehyde L87A mutant protein, pH 8.0, 25°C 714244