4.1.3.3 additional information - additional information with substrate N-acetylneuraminic acid increasing Km with increasing pH 33315 4.1.3.3 0.013 - N-acetylneuraminate purified recombinant mutant K165C, pH 7.4, 30°C 727317 4.1.3.3 0.015 - N-acetylneuraminate purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C 727317 4.1.3.3 0.023 - N-acetylneuraminate purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C 727317 4.1.3.3 0.036 - N-acetylneuraminate purified recombinant wild-type enzyme, pH 7.4, 30°C 727317 4.1.3.3 0.04 - N-acetylneuraminate purified recombinant wild-type enzyme, pH 6.8, 30°C 727317 4.1.3.3 0.2 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192M mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.2 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192P mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.27 - N-acetylneuraminate mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 0.3 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192F mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.32 - N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 0.35 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192V 666805 4.1.3.3 0.39 - (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide mutant enzyme E192N 663630 4.1.3.3 0.39 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192N 666805 4.1.3.3 0.4 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192N mutant protein, pH 7.7.5, 25°C 715921 4.1.3.3 0.4 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192V mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.4 - N-acetylneuraminic acid pH 7.2, Y133F mutant 651103 4.1.3.3 0.5 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192S 666805 4.1.3.3 0.5 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192H mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.5 - pyruvate mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 0.6 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192S mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.7 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192Q mutant protein, pH 7.5, 25°C 715921 4.1.3.3 1 - N-acetylneuraminic acid pH 7.2, Y133H mutant 651103 4.1.3.3 1 - N-acetylneuraminic acid pH 7.4, 75°C 651432 4.1.3.3 1 4 pyruvate recombinant His-taggged enzyme, pH 7.0, 37°C 727656 4.1.3.3 1.1 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192D mutant protein, pH 7.5, 25°C 715921 4.1.3.3 1.1 - N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 1.1 - pyruvate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 1.2 - N-acetylneuraminate mutant enzyme G211S, at pH 7.0 and 37°C 749150 4.1.3.3 1.4 - N-acetylneuraminic acid pH 7.4, 37°C 652833 4.1.3.3 1.5 3.7 N-acetylneuraminic acid - 650651 4.1.3.3 1.7 - N-acetylneuraminic acid pH 7.4, 37°C 651432 4.1.3.3 1.75 - N-acetylneuraminic acid - 33316 4.1.3.3 1.75 - N-Formylneuraminic acid - 33328 4.1.3.3 1.8 - N-acetylneuraminate at pH 7.0 and 37°C 748534 4.1.3.3 1.8 - N-acetylneuraminic acid - 33328 4.1.3.3 1.8 - N-acetylneuraminic acid pH 7.0, 37°C 713843 4.1.3.3 1.9 3.9 N-acetylneuraminic acid - 650651 4.1.3.3 1.93 - N-Succinylneuraminic acid - 33328 4.1.3.3 2 - N-Acetyl-8-O-acetylneuraminic acid - 33328 4.1.3.3 2 - N-acetylneuraminate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C 749150 4.1.3.3 2 - N-acetylneuraminate recombinant His-taggged enzyme, pH 7.0, 37°C 727656 4.1.3.3 2 - N-acetylneuraminic acid pH 7.2, 50 mM phosphate buffer, wild-type 651103 4.1.3.3 2 3 pyruvate - 690576 4.1.3.3 2.1 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L 666750 4.1.3.3 2.2 - N-acetylneuraminate wild-type enzyme, pH 7.4, 30°C 726551 4.1.3.3 2.2 - N-glycolylneuraminic acid - 33328 4.1.3.3 2.4 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 2.4 - N-acetylneuraminic acid - 33319 4.1.3.3 2.4 - N-acetylneuraminic acid pH 7.2, Y133C mutant 651103 4.1.3.3 2.5 - N-acetylneuraminate - 690576 4.1.3.3 2.51 - N-acetylneuraminate mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 2.54 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme S208G 666805 4.1.3.3 2.6 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 2.7 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 2.7 - N-Monochloracetylneuraminic acid - 33328 4.1.3.3 2.8 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 2.8 - N-acetyl-neuraminic acid in 0.1 M phosphate buffer, pH 7 678004 4.1.3.3 2.8 - N-acetylneuraminic acid - 33315 4.1.3.3 2.9 - N-acetylneuraminic acid pH 7.2, Y133W mutant 651103 4.1.3.3 3 3.5 N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 3 3.5 N-acetylneuraminate at pH 7.5 and 37°C 749312 4.1.3.3 3.2 - N-acetylneuraminate at 30°C, pH not specified in the publication 747776 4.1.3.3 3.2 - N-acetylneuraminate mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 3.2 - N-acetylneuraminic acid pH 7.2, 250 mM phosphate buffer, wild-type 651103 4.1.3.3 3.3 - N-acetylneuraminic acid - 33317, 33330, 33332 4.1.3.3 3.3 - N-glycolylneuraminic acid - 33332 4.1.3.3 3.4 - N-acetylneuraminate mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 3.42 - N-acetylneuraminate mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 3.5 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 3.5 - N-acetylneuraminic acid E192Q mutant protein, pH 7.5, 25°C 715921 4.1.3.3 3.5 - N-acetylneuraminic acid pH 7.2, K161R mutant 651103 4.1.3.3 3.6 - N-acetylneuraminic acid - 33314 4.1.3.3 3.7 - N-acetylneuraminate mutant Y137F enzyme, pH 7.4, 30°C 726551 4.1.3.3 3.7 - N-acetylneuraminic acid - 33321 4.1.3.3 3.9 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 3.9 - N-acetylneuraminic acid - 33328 4.1.3.3 3.93 - N-acetylneuraminate mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 4 - N-acetylneuraminate mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 4 - N-acetylneuraminate recombinant enzyme, pH and temperature not specified in the publication 727332 4.1.3.3 4.1 - N-acetylneuraminate wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 4.3 - N-acetylneuraminate mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 4.3 - N-glycolylneuraminic acid - 33314 4.1.3.3 4.3 - pyruvate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C 749150 4.1.3.3 4.4 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, wild-type enzyme 666805 4.1.3.3 4.4 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 4.4 - N-acetylneuraminic acid wild type (E192), pH 7.5, 25°C 715921 4.1.3.3 4.4 - sialic acid wild-type enzyme 663630 4.1.3.3 4.5 - N-Acetyl-7-O-acetylneuraminic acid - 33328 4.1.3.3 4.6 - N-acetylneuraminic acid E192D mutant protein, pH 7.5, 25°C 715921 4.1.3.3 4.6 - N-glycolylneuraminic acid - 33337 4.1.3.3 4.7 - N-acetylneuraminate mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 4.8 - N-acetylneuraminic acid - 33337 4.1.3.3 4.9 - N-acetylneuraminate - 690576 4.1.3.3 5 - N-acetylneuraminate mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 5 - N-acetylneuraminic acid E192M mutant protein, pH 7.5, 25°C 715921 4.1.3.3 5.7 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme D191P 666805 4.1.3.3 5.9 - N-acetylneuraminic acid E192F mutant protein, pH 7.5, 25°C 715921 4.1.3.3 5.9 - pyruvate at pH 7.0 and 37°C 748534 4.1.3.3 6 - N-acetylneuraminic acid E192P mutant protein, pH 7.5, 25°C 715921 4.1.3.3 6 - N-acetylneuraminic acid E192S mutant protein, pH 7.5, 25°C 715921 4.1.3.3 6.1 - N-acetylneuraminate mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 6.2 - N-acetylneuraminic acid E192H mutant protein, pH 7.5, 25°C 715921 4.1.3.3 6.3 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 6.9 - N-acetyl-D-mannosamine at pH 7.0 and 37°C 748534 4.1.3.3 7.5 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 7.7 - N-acetyl-D-mannosamine - 33337 4.1.3.3 8.3 - pyruvate - 33337 4.1.3.3 9 - pyruvate cross-linked enzyme aggregates, pH 7.5, 37°C 714508 4.1.3.3 9.2 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 10 - N-Monofluoracetylneuraminic acid - 33328 4.1.3.3 10.1 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 10.6 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, wild-type enzyme 666805 4.1.3.3 10.7 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme S208G 666805 4.1.3.3 11 - (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide wild-type enzyme 663630 4.1.3.3 11 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid wild type (E192), pH 7.5, 25°C 715921 4.1.3.3 11.4 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 12.1 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 12.3 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L 666750 4.1.3.3 12.5 - N-acetyl-4-O-acetylneuraminic acid - 33328 4.1.3.3 12.5 - pyruvate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 13.8 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 14.1 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L 666750 4.1.3.3 14.5 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 14.7 - pyruvate at pH 7.5 and 37°C 749312 4.1.3.3 15.3 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 17 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192S 666805 4.1.3.3 17 - N-acetylneuraminic acid pH 7.2, E188Q mutant 651103 4.1.3.3 19.9 - pyruvate pH 7.0, 37°C 713843 4.1.3.3 19.9 - pyruvate soluble enzyme, pH not specified in the publication, temperature not specified in the publication 714508 4.1.3.3 22 - pyruvate - 690576 4.1.3.3 29 - N-acetylneuraminic acid pH 7.2, D187E mutant 651103 4.1.3.3 34 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192V 666805 4.1.3.3 34 - N-acetylneuraminic acid E192V mutant protein, pH 7.5, 25°C 715921 4.1.3.3 35.1 - pyruvate recombinant enzyme, pH and temperature not specified in the publication 727332 4.1.3.3 36.2 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 38 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192N 666805 4.1.3.3 38 - N-acetylneuraminic acid E192N mutant protein, pH 7.7.5, 25°C 715921 4.1.3.3 38 - sialic acid mutant enzyme E192N 663630 4.1.3.3 44 - N-acetylneuraminic acid pH 7.2, E188D mutant 651103 4.1.3.3 53.3 - N-acetyl-D-mannosamine at pH 7.5 and 37°C 749312 4.1.3.3 72.4 - pyruvate at pH 8.5 and 37°C 749312 4.1.3.3 87.7 - N-acetylneuraminate at pH 8.5 and 37°C 749312 4.1.3.3 92.1 - N-acetyl-D-mannosamine at pH 8.5 and 37°C 749312 4.1.3.3 127 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 131.7 - N-acetyl-D-mannosamine recombinant enzyme, pH and temperature not specified in the publication 727332 4.1.3.3 149 - N-acetyl-D-mannosamine recombinant His-taggged enzyme, pH 7.0, 37°C 727656 4.1.3.3 160 - N-acetyl-D-mannosamine pH 7.0, 37°C 713843 4.1.3.3 160 - N-acetyl-D-mannosamine soluble enzyme, pH not specified in the publication, temperature not specified in the publication 714508 4.1.3.3 180 - N-acetyl-D-mannosamine - 690576 4.1.3.3 220 - N-acetyl-D-mannosamine - 690576 4.1.3.3 245 - N-acetyl-D-mannosamine cross-linked enzyme aggregates, pH 7.5, 37°C 714508 4.1.3.3 272 - N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 333 - N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 369.4 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 915 - N-acetyl-D-mannosamine mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 1100 - N-acetylneuraminic acid pH 7.2, D187N mutant 651103 4.1.3.3 3200 - N-acetyl-D-mannosamine mutant enzyme G210S/Y213G, at pH 7.0 and 37°C 749150