4.1.3.1 additional information - additional information Michaelis-Menten kinetics of wild-type and mutant enzyme's overview 728845 4.1.3.1 additional information - additional information stopped-flow spectroscopy and steady-state kinetics, solvent kinetic isotope effects, overview. Whereas the D2OV is consistent with partially rate-limiting proton transfer during formation of the hydroxyl group of isocitrate, the large inverse D2O(V/Ksuccinate) indicates that substantially different kinetic parameters exist when the enzyme is saturated with succinate 727045 4.1.3.1 0.008 - threo-Ds-isocitrate - 33115 4.1.3.1 0.014 - glyoxylate - 33137 4.1.3.1 0.015 - DL-isocitrate - 33115 4.1.3.1 0.0157 - isocitrate pH 6.5 653476 4.1.3.1 0.016 - isocitrate pH 7.5, 25°C 713812 4.1.3.1 0.018 - Ds-isocitrate pH 6.8 33116 4.1.3.1 0.018 - threo-Ds-isocitrate - 33124 4.1.3.1 0.02 - threo-Ds-isocitrate 25 mM imidazole buffer, pH 6.8 33135 4.1.3.1 0.021 - threo-Ds-isocitrate - 33133 4.1.3.1 0.025 - acetyl-CoA pH 8.0, 30°C 658479 4.1.3.1 0.026 - (2R,3S)-2,3-dimethyl malate pH 7.5, 25°C 664083 4.1.3.1 0.029 - (2R,3S)-2,3-dimethyl malate pH 7.5, 25°C 664083 4.1.3.1 0.032 - isocitrate phosphate, pH 6.0 33138 4.1.3.1 0.033 - Ds-isocitrate HEPES, pH 7.0 33123, 33138 4.1.3.1 0.035 - isocitrate imidazole, pH 7.0 33138 4.1.3.1 0.039 - isocitrate MOPS, pH 6.8 33138 4.1.3.1 0.039 - isocitrate pH 7, standard g, cleavage reaction 650574 4.1.3.1 0.04 - glyoxylate pH 8.0, 30°C 658479 4.1.3.1 0.04 - threo-Ds-isocitrate - 33140 4.1.3.1 0.042 - (2R,3S)-isocitrate pH 7.5, 25°C 664083 4.1.3.1 0.043 - isocitrate pH 7, microgravity, cleavage reaction 650574 4.1.3.1 0.044 - succinate - 33137 4.1.3.1 0.045 - DL-isocitrate isoform ICL1, with 5 mM MgCl2, at pH 7.5 and 25°C 748480 4.1.3.1 0.045 - isocitrate pH 7.5, 25°C, recombinant enzyme 727039 4.1.3.1 0.045 - isocitrate recombinant detagged enzyme, pH 7.0, 37°C 727045 4.1.3.1 0.05 - glyoxylate - 33130 4.1.3.1 0.05 - isocitrate pH 7.5, 25°C 713812 4.1.3.1 0.05 - threo-Ds-isocitrate - 33137, 33141 4.1.3.1 0.051 - threo-Ds-isocitrate - 33148 4.1.3.1 0.053 - succinate pH 7, microgravity, condensation reaction 650574 4.1.3.1 0.056 - isocitrate ICL1 702178 4.1.3.1 0.06 - DL-isocitrate at pH 7.4 and 25°C 748615 4.1.3.1 0.06 - isocitrate 100 mM Hepes, pH 7.0 33147 4.1.3.1 0.062 - Ds-isocitrate pH 7.3 33116, 33118 4.1.3.1 0.063 - DL-isocitrate isoform ICL2, with 5 mM MgCl2, at pH 7.5 and 25°C 748480 4.1.3.1 0.063 - succinate pH 7, standard g, condensation reaction 650574 4.1.3.1 0.066 - Ds-isocitrate - 33136 4.1.3.1 0.069 - (2R,3S:2S,3R)-2-methyl-isocitrate pH 7.5, 25°C 664083 4.1.3.1 0.07 - isocitrate - 33151 4.1.3.1 0.083 - isocitrate ICL2 702178 4.1.3.1 0.087 0.096 DL-isocitrate - 33146 4.1.3.1 0.088 - glyoxylate pH 7, microgravity, condensation reaction 650574 4.1.3.1 0.092 - glyoxylate pH 7, standard g, condensation reaction 650574 4.1.3.1 0.092 - Mg2+ pH 7 649454 4.1.3.1 0.093 - DL-isocitrate - 33152 4.1.3.1 0.098 - (2R)-propyl-(3S)-methyl malate pH 7.5, 25°C 664083 4.1.3.1 0.1 - isocitrate ICL 702178 4.1.3.1 0.1 - threo-Ds-isocitrate Hepes, pH 7.4 33132, 33138 4.1.3.1 0.1 0.2 isocitrate Tris, triethanolamine or imidazole buffer 33130, 33131 4.1.3.1 0.104 - (2R,3R:2S,3S)-2-methyl-isocitrate pH 7.5, 25°C 664083 4.1.3.1 0.11 - isocitrate TRA, pH 7.0 33138 4.1.3.1 0.13 - glyoxylate - 33118 4.1.3.1 0.13 - oxalacetate pH 7.5, 25°C 664083 4.1.3.1 0.136 - DL-isocitrate - 33155 4.1.3.1 0.14 - glyoxylate recombinant detagged enzyme, pH 7.0, 37°C 727045 4.1.3.1 0.145 - threo D-(s)-isocitrate Icl enzyme, 37°C 651648 4.1.3.1 0.159 - isocitrate in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C 679472 4.1.3.1 0.188 - isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 681995 4.1.3.1 0.2 - succinate - 33130 4.1.3.1 0.2 - threo-Ds-isocitrate 0.4 M KCl, 25 mM imidazole buffer, pH 6.8 33135 4.1.3.1 0.24 - succinate pH 7.5, 25°C, recombinant enzyme 727039 4.1.3.1 0.25 - Ds-isocitrate - 33125 4.1.3.1 0.25 - isocitrate 10°C 666268 4.1.3.1 0.25 - isocitrate MOPS, pH 7.5 33138 4.1.3.1 0.25 - isocitrate recombinant His-tagged mutant N214D, pH 7.8, 30°C 728845 4.1.3.1 0.26 - isocitrate mutant Q217K, 10°C 666268 4.1.3.1 0.26 - threo-Ds-isocitrate Hepes, pH 7.4, 60 mM Cl- 33132 4.1.3.1 0.29 - (2R)-2-methyl malate pH 7.5, 25°C 664083 4.1.3.1 0.29 - isocitrate phosphate, pH 7.5 33138 4.1.3.1 0.29 - isocitrate recombinant His-tagged mutant Q211H, pH 7.8, 30°C 728845 4.1.3.1 0.3 - isocitrate phosphate, pH 7.0 33138 4.1.3.1 0.3 - threo-Ds-isocitrate - 33149 4.1.3.1 0.33 - isocitrate wild-type, 10°C 666268 4.1.3.1 0.4 - glyoxylate pH 7.5, 25°C, recombinant enzyme 727039 4.1.3.1 0.412 - succinate recombinant detagged enzyme, pH 7.0, 37°C 727045 4.1.3.1 0.44 - isocitrate recombinant His-tagged wild-type enzyme, pH 7.8, 30°C 728845 4.1.3.1 0.45 - (2R)-ethyl-(3S)-methyl malate pH 7.5, 25°C 664083 4.1.3.1 0.47 - isocitrate recombinant His-tagged mutant Q221K, pH 7.8, 30°C 728845 4.1.3.1 0.47 - isocitrate wild-type, 20°C 666268 4.1.3.1 0.5 - isocitrate mutant Q217K, 30°C 666268 4.1.3.1 0.51 - DL-isocitrate 20°C, pH 6.8 638504 4.1.3.1 0.51 - isocitrate 50 mM MOPS buffer, pH 7.5, with 1 mM MgCl2, 10 mM 2-mercaptoethanol, and 2.7 mM O-nitrophenyl-beta-D-galactopyranoside 679978 4.1.3.1 0.52 - isocitrate 60 mM potassium phosphate, pH 7.5 33147 4.1.3.1 0.5287 - isocitrate mutant enzyme T534A, at pH 7.0 and 37°C 748505 4.1.3.1 0.5287 - isocitrate wild type enzyme, at pH 7.0 and 37°C 748505 4.1.3.1 0.53 - (2R)-ethyl-(3S)-methyl malate pH 7.5, 25°C 664083 4.1.3.1 0.5433 - isocitrate mutant enzyme T534V, at pH 7.0 and 37°C 748505 4.1.3.1 0.59 - succinate - 33118 4.1.3.1 0.6 - DL-isocitrate - 33149 4.1.3.1 0.62 - DL-isocitrate imidazole-HCl buffer pH 6.85 33153 4.1.3.1 0.633 - DL-isocitrate 15°C, pH 6.8, Km increases with elevating temperature 651215 4.1.3.1 0.65 - isocitrate 40°C 666268 4.1.3.1 0.66 - isocitrate Tris, pH 7.6 33138 4.1.3.1 0.67 - isocitrate phosphate, pH 7.5 33138 4.1.3.1 0.718 - (2R,3S:2S,3R)-2-methyl-isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 681995 4.1.3.1 0.72 - isocitrate - 679815 4.1.3.1 0.76 - isocitrate 60 mM triethanolamine, pH 7.5 33147 4.1.3.1 0.76 - isocitrate mutant Q207H, 10°C 666268 4.1.3.1 0.94 - isocitrate recombinant His-tagged mutant E219A, pH 7.8, 30°C 728845 4.1.3.1 0.96 - glyoxylate D475N mutant protein, 0.15 mM acetyl-CoA, 10 mM succinate, pH 6.5, 30°C 715749 4.1.3.1 0.96 - isocitrate pH 7.5, 70°C, recombinant enzyme 715548 4.1.3.1 1 - isocitrate phosphate buffer, pH 6.8 or 7.5 33130, 33131 4.1.3.1 1 - isocitrate phosphate, pH 6.8 33138 4.1.3.1 1.1 - (2R)-2-ethyl malate pH 7.5, 25°C 664083 4.1.3.1 1.1 - succinate - 33126 4.1.3.1 1.14 - isocitrate ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 681995 4.1.3.1 1.142 - threo-DL-Isocitrate wild type enzyme, at pH 7.5 and 30°C 747203 4.1.3.1 1.16 - D-isocitrate pH 7.0, 40°C 727500 4.1.3.1 1.2 1.7 DL-isocitrate - 33120 4.1.3.1 1.3 - glyoxylate D475N mutant protein, 10 mM succinate, pH 6.5, 30°C 715749 4.1.3.1 1.3 - threo D-(s)-isocitrate AceA enzyme, Tricine buffer, 37°C, pH 7.5 651648 4.1.3.1 1.4 - isocitrate pH 7.5, 25°C 664147 4.1.3.1 1.423 - threo-DL-Isocitrate mutant enzyme E423D, at pH 7.5 and 30°C 747203 4.1.3.1 1.532 - DL-isocitrate sodium phosphate buffer, pH 6.85 33153 4.1.3.1 1.6 - DL-isocitrate pH 7 649454 4.1.3.1 1.861 - threo-DL-Isocitrate mutant enzyme E424D, at pH 7.5 and 30°C 747203 4.1.3.1 2.031 - threo-DL-Isocitrate mutant enzyme E423D/E424D, at pH 7.5 and 30°C 747203 4.1.3.1 2.07 - isocitrate mutant Q207H, 30°C 666268 4.1.3.1 6 - succinate D475N mutant protein, 1.5 mM glyoxylate, sharply decreased with increasing acetyl-CoA concentration, pH 6.5, 30°C 715749 4.1.3.1 6.7 - isocitrate when acetyl-CoA is present at concentrations higher than 5 mM, the Km value for isocitrate decreased by about one-third and the Vmax value increased about 2-fold, pH not specified in the publication, temperature not specified in the publication 715749 4.1.3.1 7.6 - isocitrate pH 6.5, 30°C 658479 4.1.3.1 8 - (2R)-isobutyl-(3S)-methyl malate pH 7.5, 25°C 664083