4.1.2.52 additional information - additional information apparent Km values for Co2+ increase in the two mutants H45A and H45Q by about 800fold compared to the wild-type enzyme 722258 4.1.2.52 0.006 - 4-hydroxy-2-oxopentanoate Km (app) with Co2+, pH 8.0, 25°C 721601 4.1.2.52 0.0175 - 4-hydroxy-2-oxopentanoate Km (app) with Mn2+, pH 8.0, 25°C 721601 4.1.2.52 0.05 - 2-oxo-3-methyl-4-hydroxypentanoate wild type enzyme, at pH 8.0 and 25°C 725493 4.1.2.52 0.16 - 4-hydroxy-2-oxohexanoate pH 8.0, 25°C 721601 4.1.2.52 0.289 - 4-hydroxy-2-oxopentanoate Km (app) with Mg2+, pH 8.0, 25°C 721601 4.1.2.52 0.35 - 4-hydroxy-2-oxoheptane-1,7-dioate using H2O as solvent, in the presence of 0.5 mM Co2+, at pH 8.0 and 25°C 725493 4.1.2.52 0.38 - (S)-4-hydroxy-2-oxopentanoate wild type enzyme, with 0.5 mM Co2+, at pH 8.0 and 25°C 725493 4.1.2.52 0.38 - 4-hydroxy-2-oxopentanoate pH 8.0, 25°C 721601 4.1.2.52 0.38 - 4-hydroxy-2-oxopentanoic acid wild-type, pH 8.0, 25°C 722258 4.1.2.52 3.32 - (S)-4-hydroxy-2-oxopentanoate mutant enzyme R70K, with 0.5 mM Co2+, at pH 8.0 and 25°C 725493 4.1.2.52 5.6 - pyruvate steady-state kinetic parameter, pH 8.0 and 25°C 702377 4.1.2.52 9.1 - Succinic semialdehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 13.4 - Butyraldehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 14.22 - 3-deoxy-D-manno-oct-2-ulosonic acid i.e. KDO, pH 8.0, 25°C 721601 4.1.2.52 16 - 4-hydroxy-2-oxopentanoic acid H45Q mutant enzyme, pH 8.0, 25°C 722258 4.1.2.52 32.9 - propionaldehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 33.3 - glycolaldehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 38 - 4-hydroxy-2-oxopentanoic acid H45A mutant enzyme, pH 8.0, 25°C 722258 4.1.2.52 50.1 - acetaldehyde Km(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 62.1 - acetaldehyde steady-state kinetic parameter, pH 8.0 and 25°C 702377 4.1.2.52 62.9 - acetaldehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 73.8 - Isobutyraldehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377 4.1.2.52 88.6 - DL-glyceraldehyde Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C 702377