4.1.2.17 additional information - additional information for FucA catalysis, the rate of the aldol addition reaction of dihydroxyaceone phosphate to N-Cbz-amino aldehydes in borate is between 2 to 10 times faster than that inTEA buffer. Moreover, the yields of aldol adduct improve between 1.5 to 4-fold for both FucA wild-type and the F131A mutant 728423 4.1.2.17 0.091 - dihydroxyacetone phosphate pH and temperature not specified in the publication 727377 4.1.2.17 0.091 - dihydroxyacetone phosphate pH not specified in the publication, temperature not specified in the publication 727377 4.1.2.17 0.36 - glycerone phosphate N25L mutant 652766 4.1.2.17 0.5 - L-Fuculose 1-phosphate pH 7.5, 37°C, Y113F mutant 649990 4.1.2.17 0.7 - L-Fuculose 1-phosphate - 5047, 5048, 5049 4.1.2.17 0.743 - D-glyceraldehyde assay with DL-glyceraldehyde, pH and temperature not specified in the publication 727377 4.1.2.17 0.743 - DL-glyceraldehyde pH not specified in the publication, temperature not specified in the publication 727377 4.1.2.17 0.743 - L-glyceraldehyde assay with DL-glyceraldehyde, pH and temperature not specified in the publication 727377 4.1.2.17 1.01 - glycerone phosphate wild-type 652766 4.1.2.17 1.8 - L-Fuculose 1-phosphate pH 7.5, 37°C, R212A mutant 649990 4.1.2.17 2 - L-Fuculose 1-phosphate pH 7.5, 37°C, wild-type enzyme 649990 4.1.2.17 2.2 - L-Fuculose 1-phosphate pH 7.5, 37°C, wild-type 652834 4.1.2.17 3 - L-Fuculose 1-phosphate pH 7.5, 37°C, E214D mutant 649990 4.1.2.17 3 - L-Fuculose 1-phosphate pH 7.5, 37°C, E215A mutant 649990 4.1.2.17 4.4 - L-Fuculose 1-phosphate pH 7.5, 37°C, Y209F mutant 649990 4.1.2.17 5 - glycerone phosphate N25T mutant 652766 4.1.2.17 6 - L-Fuculose 1-phosphate pH 7.5, 37°C, E214Q and T26A mutant 649990 4.1.2.17 7 - L-Fuculose 1-phosphate pH 7.5, 37°C, K207A mutant 649990 4.1.2.17 7.8 - DL-glyceraldehyde wild-type 652766 4.1.2.17 8 - L-Fuculose 1-phosphate pH 7.5, 37°C, E214A mutant 649990 4.1.2.17 8.2 - DL-glyceraldehyde N25T mutant 652766 4.1.2.17 11 - L-Fuculose 1-phosphate pH 7.5, 37°C, F206W mutant 649990 4.1.2.17 22 - L-Fuculose 1-phosphate pH 7.5, 37°C, F131A mutant 649990 4.1.2.17 23.1 - DL-glyceraldehyde N25L mutant 652766 4.1.2.17 54.7 - dihydroxyacetone phosphate - 690787 4.1.2.17 74 - L-Fuculose 1-phosphate pH 7.5, 37°C, E214L mutant 649990 4.1.2.17 130 - L-Fuculose 1-phosphate pH 7.5, 37°C, N29Q mutant 652834