4.1.1.112 additional information - additional information kinetic data 4269, 4275 4.1.1.112 additional information - additional information positive cooperativity for oxaloacetate 4269 4.1.1.112 0.0003 - oxaloacetate wild-type, Vmax = 4 micromol/min/mg 692317 4.1.1.112 0.0003 - oxaloacetate Y235A mutant, Vmax = 0.2 micromol/min/mg. In the presence of GDP 692317 4.1.1.112 0.0003 - oxaloacetate Y235F mutant, Vmax = 2 micromol/min/mg 692317 4.1.1.112 0.0003 - oxaloacetate Y235S mutant, Vmax = 0.1 micromol/min/mg. In the presence of GDP 692317 4.1.1.112 0.0004 - oxaloacetate wild-type, Vmax = 3 micromol/min/mg. In the presence of GDP 692317 4.1.1.112 0.0004 - oxaloacetate Y235F mutant, Vmax = 3 micromol/min/mg. In the presence of GDP 692317 4.1.1.112 0.03 - oxaloacetate pH 7.1 653877 4.1.1.112 0.032 - oxaloacetate at pH 7.4 and 25°C 748206 4.1.1.112 0.06 - oxaloacetate - 4263 4.1.1.112 0.087 - oxaloacetate mutant enzyme Y212F, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C 678331 4.1.1.112 0.15 - oxaloacetate - 4271 4.1.1.112 0.22 - oxaloacetate - 4268 4.1.1.112 0.23 - oxaloacetate mitochondrial enzyme 4271 4.1.1.112 0.52 - oxaloacetate - 664936 4.1.1.112 0.55 - oxaloacetate - 4270 4.1.1.112 0.62 - oxaloacetate calculated value at pH 4.5, temperature not specified in the publication 714979 4.1.1.112 0.63 - 3-methyloxaloacetate wild type enzyme, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C 678331 4.1.1.112 1 - CO2 - 4263 4.1.1.112 1.02 - oxaloacetate mutant enzyme H235A, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C 678331 4.1.1.112 1.39 - oxaloacetate in 100 mM Tris-HCl (pH 7.3), 10 mM MgCl2, 2.5 mM EDTA, at 30°C 704327 4.1.1.112 1.7 - oxaloacetate - 4283 4.1.1.112 2.1 - oxaloacetate - 4276 4.1.1.112 2.1 - oxaloacetate pH 8.0, 25°C 654328 4.1.1.112 2.2 - oxaloacetate wild type enzyme, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C 678331 4.1.1.112 2.5 - oxaloacetate mutant enzyme H235Q, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C 678331 4.1.1.112 3.3 - pyruvate - 4263 4.1.1.112 4.3 - oxaloacetate - 4272 4.1.1.112 20.7 - oxaloacetate pH 7.4, 25°C, wild-type enzyme 756006